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Literature summary extracted from

  • Esders, T.W.; Michrina, C.A.
    Purification and properties of L-alpha-glycerophosphate oxidase from Streptococcus faecium ATCC 12755 (1979), J. Biol. Chem., 254, 2710-2715.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.21 additional information increasing the buffer concentration from 10 mM to 100 mM leads to an 2.6-10fold increase in activity, this effect is observed with potassium phosphate, sodium acetate, sodium maleate or other anionic buffers Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.21 fructose-1-phosphate
-
 Enterococcus faecium
1.1.3.21 fructose-6-phosphate
-
 Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.21 26
-
 sn-glycerol-3-phosphate
-
 Enterococcus faecium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.21 CaCl2 10 mM, 7.7fold activation Enterococcus faecium
1.1.3.21 CoCl2 10 mM, 10.2fold activation Enterococcus faecium
1.1.3.21 KCl 100 mM, 5.8fold activation Enterococcus faecium
1.1.3.21 MgCl2 10 mM, 6.8fold activation Enterococcus faecium
1.1.3.21 MnCl2 10 mM, 7.6 fold activation Enterococcus faecium
1.1.3.21 NaCl 100 mM, 3.8fold activation Enterococcus faecium
1.1.3.21 ZnCl2 1 mM, 6.3fold activation Enterococcus faecium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.21 72000
-
 2 * 72000, SDS-PAGE Enterococcus faecium
1.1.3.21 131000
-
 strain ATCC 12755, gel filtration, sucrose density centrifugation Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.21 sn-glycerol-3-phosphate + O2 Enterococcus faecium
-
 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.21 Enterococcus faecium
-
 ATCC 12755
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.21
-
 Enterococcus faecium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.21 85 95 strain ATCC 12755 Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.21 sn-glycerol-3-phosphate + O2
-
 Enterococcus faecium glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
1.1.3.21 sn-glycerol-3-phosphate + O2 high specificity for L-alpha-glycerophosphate Enterococcus faecium glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Subunits

EC Number Subunits Comment Organism
1.1.3.21 dimer 2 * 72000, SDS-PAGE Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.21 7
-
-
 Enterococcus faecium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.21 FAD prosthetic group Enterococcus faecium
1.1.3.21 FAD 2 mol bound per mol of enzyme Enterococcus faecium