Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jones, J.M.; Morrell, J.C.; Gould, S.J.
    Identification and characterization of HAOX1, HAOX2, and HAOX3, three human peroxisomal 2-hydroxy acid oxidases (2000), J. Biol. Chem., 275, 12590-12597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.98 three different genes, HAOX1, HAOX2, HAOX3, coding for 3 different 2-hydroxy acid oxidases, overexpression in Escherichia coli strain DH10B as His-tagged protein and in human skin fibroblasts GM5756-T cells as c-myc-tagged protein Homo sapiens
1.1.1.99 three different genes, HAOX1, HAOX2, HAOX3, coding for 3 different 2-hydroxy acid oxidases, overexpression in Escherichia coli strain DH10B as His-tagged protein and in human skin fibroblasts GM5756-T cells as c-myc-tagged protein Homo sapiens
1.1.3.15 expression in Escherichia coli, expression in human skin fibroblasts Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.98 additional information overexpression of HAOX1, HAOX2, HAOX3 in Escherichia coli strain DH10B as His-tagged proteins and in human skin fibroblasts GM5756-T cells as c-myc-tagged proteins Homo sapiens
1.1.1.99 additional information overexpression of HAOX1, HAOX2, HAOX3 in Escherichia coli strain DH10B as His-tagged proteins and in human skin fibroblasts GM5756-T cells as c-myc-tagged proteins Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.045
-
 2-Hydroxyoctanoate isoenzyme HAOX3 Homo sapiens
1.1.3.15 0.12
-
 glycolate isoenzyme HAOX1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.98 peroxisome
-
 Homo sapiens 5777
-
1.1.1.99 peroxisome
-
 Homo sapiens 5777
-
1.1.3.15 peroxisome
-
 Homo sapiens 5777
-
1.1.3.15 peroxisome
-
 Rattus sp. 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 2-hydroxyoctanoate + O2 Homo sapiens substrates for isoenzymes HAOX1, HAOX2, preferred substrate for isoenzyme HAOX3 2-oxooctanoate + H2O2
-
 ?
1.1.3.15 2-hydroxypalmitate + O2 Homo sapiens substrates for isoenzymes HAOX1, HAOX2 2-oxopalmitate + H2O2
-
 ?
1.1.3.15 glycolate + O2 Rattus sp.
-
 glyoxylate + H2O2
-
 ?
1.1.3.15 glycolate + O2 Homo sapiens highest activity for isoenzyme HAOX1, no activity for isoenzymes HAOX2, HAOX3 glyoxylate + H2O2
-
 ?
1.1.3.15 glyoxylate + O2 Rattus sp.
-
 ? + H2O2
-
 ?
1.1.3.15 glyoxylate + O2 Homo sapiens substrate for isoenzyme HAOX1 ? + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.98 Homo sapiens
-
 three different forms: HAOX1, HAOX2, HAOX3
-
1.1.1.99 Homo sapiens
-
 three different forms: HAOX1, HAOX2, HAOX3
-
1.1.3.15 Homo sapiens
-
-
-
1.1.3.15 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.98 His-tagged protein Homo sapiens
1.1.1.99 His-tagged protein Homo sapiens
1.1.3.15 recombinant isoenzymes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.98 kidney HAOX2 Homo sapiens
-
1.1.1.98 liver HAOX1 and HAOX2 Homo sapiens
-
1.1.1.98 pancreas HAOX1 and, exclusively, HAOX3 Homo sapiens
-
1.1.1.99 kidney HAOX2 Homo sapiens
-
1.1.1.99 liver HAOX1 and HAOX2 Homo sapiens
-
1.1.1.99 pancreas HAOX1 and, exclusively, HAOX3 Homo sapiens
-
1.1.3.15 kidney
-
 Rattus sp.
-
1.1.3.15 kidney distribution of isoenzyme HAOX2 Homo sapiens
-
1.1.3.15 liver
-
 Rattus sp.
-
1.1.3.15 liver distribution of isoenzymes HAOX1, HAOX2 Homo sapiens
-
1.1.3.15 pancreas distribution of isoenzymes HAOX1, HAOX3 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 2-hydroxyoctanoate + O2 substrates for isoenzymes HAOX1, HAOX2, preferred substrate for isoenzyme HAOX3 Homo sapiens 2-oxooctanoate + H2O2
-
 ?
1.1.3.15 2-hydroxypalmitate + O2 substrates for isoenzymes HAOX1, HAOX2 Homo sapiens 2-oxopalmitate + H2O2
-
 ?
1.1.3.15 glycolate + O2
-
 Rattus sp. glyoxylate + H2O2
-
 ?
1.1.3.15 glycolate + O2 highest activity for isoenzyme HAOX1, no activity for isoenzymes HAOX2, HAOX3 Homo sapiens glyoxylate + H2O2
-
 ?
1.1.3.15 glyoxylate + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 glyoxylate + O2 substrate for isoenzyme HAOX1 Homo sapiens ? + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
 Homo sapiens
1.1.3.15 FMN
-
 Rattus sp.