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Literature summary extracted from

  • Ho, C.L.; Noji, M.; Saito, M.; Saito, K.
    Regulation of serine biosynthesis in Arabidopsis (1999), J. Biol. Chem., 274, 397-402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.95 overexpression in Escherichia coli strain AD494 (DE3), cloning of GFP-mutant with transit peptide, expression in plant Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.95 additional information expression of GFP-mutant enzyme in Arabidopsis to investigate the tissue distribution, complementation of Escherichia coli serA- mutant Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.95 additional information Ser, Tyr, Val, Gly, Trp, O-acetyl-L-Ser, and Cys have no effect on enzyme activity in both directions Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.95 0.01
-
 NAD+ pH 9.0 Arabidopsis thaliana
1.1.1.95 0.12
-
 NADH pH 7.1 Arabidopsis thaliana
1.1.1.95 0.35
-
 3-phosphohydroxypyruvate pH 7.1 Arabidopsis thaliana
1.1.1.95 1.19
-
 3-phospho-D-glycerate pH 9.0 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.95 chloroplast in leaves Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.95 66453
-
 x * 66453 and 90000, DNA sequence analysis and SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.95 3-phosphoglycerate + NAD+ Arabidopsis thaliana first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate 3-phosphohydroxypyruvate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.95 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.95
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.95 leaf light-grown more than dark-grown Arabidopsis thaliana
-
1.1.1.95 root light-grown more than dark-grown Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.95 0.14
-
 3-phosphoglycerate oxidation, recombinant protein Arabidopsis thaliana
1.1.1.95 10.95
-
 3-phosphohydroxypyruvate reduction, recombinant protein Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Arabidopsis thaliana 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.1.95 3-phosphoglycerate + NAD+
-
 Arabidopsis thaliana 3-phosphohydroxypyruvate + NADH
-
 r
1.1.1.95 3-phosphoglycerate + NAD+ first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate Arabidopsis thaliana 3-phosphohydroxypyruvate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.95 ? x * 66453 and 90000, DNA sequence analysis and SDS-PAGE Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.95 NAD+
-
 Arabidopsis thaliana
1.1.1.95 NADH
-
 Arabidopsis thaliana