EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.95 | overexpression of serA gene, that codes for the enzyme, in Escherichia coli strain JM105 | Escherichia coli |
1.1.1.399 | expressed in Escherichia coli JM105 cells | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.95 | - |
Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.95 | 3-phosphoglycerate | - |
Escherichia coli | |
1.1.1.95 | glycine | - |
Escherichia coli | |
1.1.1.95 | hydroxyglutarate | product inhibition of the alpha-ketoglutarate reduction | Escherichia coli | |
1.1.1.95 | L-serine | allosteric inhibition, regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364 | Escherichia coli | |
1.1.1.399 | 3-phosphoglycerate | noncompetitive inhibition | Escherichia coli | |
1.1.1.399 | D-2-hydroxyglutaric acid | competitive inhibition | Escherichia coli | |
1.1.1.399 | glycine | - |
Escherichia coli | |
1.1.1.399 | L-serine | noncompetitive inhibition | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.95 | 0.0032 | - |
3-phosphohydroxypyruvate | apparent | Escherichia coli | |
1.1.1.95 | 0.088 | - |
alpha-ketoglutarate | apparent | Escherichia coli | |
1.1.1.399 | 0.088 | - |
2-oxoglutarate | at pH 7.5 and 37°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.399 | 46000 | - |
x * 46000, SDS-PAGE | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.95 | 3-phosphoglycerate + NAD+ | Escherichia coli | allosteric inhibition by L-serine, L-serine regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364, 1 serine binds per subunit | 3-phosphohydroxypyruvate + NADH | - |
r | |
1.1.1.399 | (R)-2-hydroxyglutarate + NAD+ | Escherichia coli | - |
2-oxoglutarate + NADH + H+ | - |
r | |
1.1.1.399 | 2-oxoglutarate + NADH + H+ | Escherichia coli | - |
(R)-2-hydroxyglutarate + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.95 | Escherichia coli | - |
- |
- |
1.1.1.399 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.95 | - |
Escherichia coli |
1.1.1.399 | DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and 5'-AMP-Sepharose affinity column chromatography | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.95 | 3-phospho-D-glycerate + NAD+ = 3-phosphooxypyruvate + NADH + H+ | member of the 2-hydroxyacid dehydrogenases family, whereof only the enzymes from E. coli and Pisum sativum utilize phosphorylated substrates and transfer the hydride ion to the A-site of NAD+, uses also alpha-ketoglutarate as substrate | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 8.7 | - |
3-phosphohydroxypyruvate reduction | Escherichia coli |
1.1.1.95 | 9.7 | - |
alpha-ketoglutarate reduction | Escherichia coli |
1.1.1.399 | 3.27 | - |
crude extract, at pH 7.5 and 37°C | Escherichia coli |
1.1.1.399 | 9.7 | - |
after 3fold purification, at pH 7.5 and 37°C | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.95 | -75°C, 15% glycerol, purified stable for months | Escherichia coli |
1.1.1.95 | 4°C, purified stable for weeks | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.95 | 3-phosphoglycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r | |
1.1.1.95 | 3-phosphoglycerate + NAD+ | allosteric inhibition by L-serine, L-serine regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364, 1 serine binds per subunit | Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r | |
1.1.1.95 | alpha-ketoglutarate + NADH | - |
Escherichia coli | 2-hydroxyglutaric acid + NAD+ | both D- and L-isomer serve as substrate for the reverse reaction, but L-isomer is a poor substrate and probably due to contamination | r | |
1.1.1.399 | (R)-2-hydroxyglutarate + NAD+ | - |
Escherichia coli | 2-oxoglutarate + NADH + H+ | - |
r | |
1.1.1.399 | 2-oxoglutarate + NADH + H+ | - |
Escherichia coli | (R)-2-hydroxyglutarate + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.399 | ? | x * 46000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.399 | alphaKG reductase | - |
Escherichia coli |
1.1.1.399 | serA | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 45 | 50 | reduction, but enzyme is thermally unstable, therefore assay is performed at 37°C | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 45 | - |
3 min, 40% loss of reductase activity | Escherichia coli |
1.1.1.399 | - |
40 | the enzyme remains stable after 3 min incubation between 0 and 40°C, while 3 min at 45, 50 and 60°C leads to about 40, 70, and 100% loss of activity | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.95 | 0.463 | - |
3-phosphohydroxypyruvate | apparent | Escherichia coli | |
1.1.1.95 | 0.555 | - |
alpha-ketoglutarate | apparent | Escherichia coli | |
1.1.1.399 | 33.3 | - |
2-oxoglutarate | at pH 7.5 and 37°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 8.5 | - |
3-phosphohydroxypyruvate and alpha-ketoglutarate reduction, enzyme and substrate not stable, product inhibition | Escherichia coli |
1.1.1.95 | 9.5 | - |
oxidation of D-3-phosphoglycerate | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 6 | 8.5 | activity drops sharply above pH 8.5 | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.95 | NAD+ | hydride ion is transferred to A-site | Escherichia coli | |
1.1.1.95 | NADH | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.399 | 0.0054 | - |
L-serine | at pH 7.5 and 37°C | Escherichia coli | |
1.1.1.399 | 0.37 | - |
D-2-hydroxyglutaric acid | at pH 7.5 and 37°C | Escherichia coli | |
1.1.1.399 | 12.7 | - |
3-phosphoglycerate | at pH 7.5 and 37°C | Escherichia coli |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.399 | 0.0076 | - |
at pH 7.5 and 37°C | Escherichia coli | L-serine |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.399 | 380 | - |
2-oxoglutarate | at pH 7.5 and 37°C | Escherichia coli |