EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.82 | thioredoxin | activated by thiol/disulfide interchange with reduced thioredoxin | Sorghum sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.82 | C207A | strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate | Sorghum sp. |
1.1.1.82 | C207A/DETAEV | very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate | Sorghum sp. |
1.1.1.82 | C207A/E387Q | very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate | Sorghum sp. |
1.1.1.82 | C29S | strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate | Sorghum sp. |
1.1.1.82 | C29S/C207A | no spontaneous activity, activated much faster than the wild-type protein, strictly dependent on reduced thioredoxin for activation | Sorghum sp. |
1.1.1.82 | C29S/C207A/DELTAEV | high spontaneous activity, activated by reduced thioredoxin almost instantaneously and also by dithiothreitol alone, although at a much slower rate. The Km-values for both the oxidized and reduced enzyme show no significant differences in the apparent affinity for NADPH, whereas the Km for oxaloacetate is dramatically increased in the oxidized form | Sorghum sp. |
1.1.1.82 | C29S/C207A/E387Q | high spontaneous activity, activated by reduced thioredoxin almost instantaneously and also by dithiothreitol alone, although at a much slower rate. The Km-values for both the oxidized and reduced enzyme show no significant differences in the apparent affinity for NADPH, whereas the Km for oxaloacetate is dramatically increased in the oxidized form | Sorghum sp. |
1.1.1.82 | C29S/DELTAEV | very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate | Sorghum sp. |
1.1.1.82 | C39S/E387Q | very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate | Sorghum sp. |
1.1.1.82 | DELTAEV | mutant with the two most C-terminal residues deleted, NADP+ does not inhibit activation, activation time course of thioredoxin-dependent activation of both mutant proteins is similar to that of the wild-type protein | Sorghum sp. |
1.1.1.82 | E387Q | NADP+ does not inhibit activation, activation time course of thioredoxin-dependent activation of both mutant proteins is similar to that of the wild-type protein | Sorghum sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.82 | 0.029 | - |
NADPH | mutant enzyme C207A/E387Q, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.031 | - |
NADPH | mutant enzyme C29S/C207A/E387Q, oxidized enzyme form | Sorghum sp. | |
1.1.1.82 | 0.035 | - |
NADPH | mutant enzyme C29S/C207A/DELTAEV, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.044 | - |
NADPH | mutant enzyme C29S/E387Q, reduced form | Sorghum sp. | |
1.1.1.82 | 0.046 | - |
NADPH | mutant enzyme C29S/E387Q, reduced form | Sorghum sp. | |
1.1.1.82 | 0.047 | - |
NADPH | mutant enzyme C207A/DELTAEV, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.049 | - |
NADPH | mutant enzyme C29S/DELTAEV, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.05 | - |
oxaloacetate | mutant enzyme C39S/E387Q, reduced form | Sorghum sp. | |
1.1.1.82 | 0.054 | - |
oxaloacetate | mutant enzyme C207A/DELTAEV, reduced form | Sorghum sp. | |
1.1.1.82 | 0.056 | - |
oxaloacetate | mutant enzyme C29S/E387Q, reduced form | Sorghum sp. | |
1.1.1.82 | 0.06 | - |
NADPH | mutant enzyme C207A/DELTAEV, and mutant enzyme C29S/C207S/DELTAEV, reduced forms | Sorghum sp. | |
1.1.1.82 | 0.063 | - |
NADPH | mutant enzyme C29S/DELTAEV, reduced form | Sorghum sp. | |
1.1.1.82 | 0.066 | - |
NADPH | mutant enzyme C29S/C207A/E387Q, reduced form and mutant enzyme C29S/C207A/DELTAEV, reduced form | Sorghum sp. | |
1.1.1.82 | 0.07 | - |
oxaloacetate | mutant enzyme C29S/DELTAEV, reduced form | Sorghum sp. | |
1.1.1.82 | 0.086 | - |
oxaloacetate | mutant enzyme C29S/C207A/E387Q, reduced form | Sorghum sp. | |
1.1.1.82 | 0.094 | - |
oxaloacetate | mutant enzyme C29S/C207A, reduced form | Sorghum sp. | |
1.1.1.82 | 0.115 | - |
NADPH | mutant enzyme C29S/C207A, reduced form | Sorghum sp. | |
1.1.1.82 | 0.447 | - |
oxaloacetate | mutant enzyme C207A/DELTAEV, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.578 | - |
oxaloacetate | mutant enzyme C29S/DELTAEV, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.583 | - |
oxaloacetate | mutant enzyme C29S/E387Q, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.894 | - |
oxaloacetate | mutant enzyme C29S/C207A/E387Q, oxidized form | Sorghum sp. | |
1.1.1.82 | 0.931 | - |
oxaloacetate | mutant enzyme C29S/C207A/DELTAEV, oxidized form | Sorghum sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.82 | chloroplast | - |
Sorghum sp. | 9507 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.82 | Sorghum sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.82 | oxaloacetate + NADPH | - |
Sorghum sp. | (S)-malate + NADP+ | - |
? |