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Literature summary extracted from

  • Issakidis, E.; Miginiac-Maslow, M.; Decottignies, P.; Jacquot, J.P.; Cretin, C.; Gadal, P.
    Site-directed mutagenesis reveals the involvement of an additional thioredoxin-dependent regulatory site in the activation of recombinant sorghum leaf NADP-malate dehydrogenase (1992), J. Biol. Chem., 267, 21577-21583.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.82 additional information thioredoxin-dependent reduction steps are involved in NADP-dependent malate dehydrogenase light activation Sorghum sp.
1.1.1.82 thioredoxin thioredoxin-dependent reduction steps are involved in NADP-dependent malate dehydrogenase light activation Sorghum sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.82 high-level expression in Escherichia coli, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S and DELTAN, a truncation mutant corresponding to the deletion of the 5' end of the mdh cDNA open reading frame until the 73rd codon Sorghum sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.82 C64S still requires activation by reduced thioredoxin, activation is almost instantaneous, whereas the native enzyme reaches full activity after 10-20 min of preincubation, the half-saturation concentration for reduced thioredoxin is decreased 2fold Sorghum sp.
1.1.1.82 C64S/C69S still requires activation by reduced thioredoxin, activation is almost instantaneous, whereas the native enzyme reaches full activity after 10-20 min of preincubation, the half-saturation concentration for reduced thioredoxin is decreased 2fold Sorghum sp.
1.1.1.82 C69S still requires activation by reduced thioredoxin, activation is almost instantaneous, whereas the native enzyme reaches full activity after 10-20 min of preincubation, the half-saturation concentration for reduced thioredoxin is decreased 2fold Sorghum sp.
1.1.1.82 DELTAN truncation mutant corresponding to the deletion of the 5' end of the mdh cDNA open reading frame until the 73rd codon Sorghum sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.82 0.022
-
 oxaloacetate mutant enzyme DELTAN Sorghum sp.
1.1.1.82 0.028
-
 NADPH mutant enzyme C64S/C69S Sorghum sp.
1.1.1.82 0.037
-
 oxaloacetate mutant enzyme C64S Sorghum sp.
1.1.1.82 0.037
-
 NADPH mutant C69S Sorghum sp.
1.1.1.82 0.038
-
 NADPH mutant enzyme C64S Sorghum sp.
1.1.1.82 0.039
-
 NADPH mutant enzyme DELTAN Sorghum sp.
1.1.1.82 0.041
-
 oxaloacetate wild-type enzyme Sorghum sp.
1.1.1.82 0.042
-
 NADPH wild-type enzyme Sorghum sp.
1.1.1.82 0.042
-
 oxaloacetate mutant C69S Sorghum sp.
1.1.1.82 0.044
-
 oxaloacetate mutant enzyme C64S/C69S Sorghum sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.82 chloroplast
-
 Sorghum sp. 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.82 33000
-
 x * 33000, DELTAN, a truncation mutant corresponding to the deletion of the 5 end of the mdh cDNA open reading frame until the 73rd codon, SDS-PAGE Sorghum sp.
1.1.1.82 40000
-
 x * 40000, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S, SDS-PAGE Sorghum sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.82 Sorghum sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.82
-
 Sorghum sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.82 leaf
-
 Sorghum sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.82 oxaloacetate + NADPH
-
 Sorghum sp. (S)-malate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.82 ? x * 40000, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S, SDS-PAGE Sorghum sp.
1.1.1.82 ? x * 33000, DELTAN, a truncation mutant corresponding to the deletion of the 5' end of the mdh cDNA open reading frame until the 73rd codon, SDS-PAGE Sorghum sp.