BRENDA - Enzyme Database

Properties of NADP+-malate dehydrogenase from immature pod wall of Cicer arietinum

Gupta, V.K.; Singh, R.; Plant Physiol. Biochem. 28, 671-678 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.82
2-oxoglutarate
5 mM
Cicer arietinum
1.1.1.82
ATP
5 mM
Cicer arietinum
1.1.1.82
citrate
5 mM
Cicer arietinum
1.1.1.82
isocitrate
5 mM
Cicer arietinum
1.1.1.82
malate
5 mM
Cicer arietinum
1.1.1.82
NADP+
5 mM
Cicer arietinum
1.1.1.82
oxalate
5 mM
Cicer arietinum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.82
0.011
-
NADPH
-
Cicer arietinum
1.1.1.82
0.029
-
NADP+
-
Cicer arietinum
1.1.1.82
0.062
-
oxaloacetate
-
Cicer arietinum
1.1.1.82
2
-
(S)-malate
-
Cicer arietinum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.82
135000
-
gel filtration
Cicer arietinum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.82
oxaloacetate + NADPH
Cicer arietinum
under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis
(S)-malate + NADP+
-
Cicer arietinum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.82
Cicer arietinum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.82
-
Cicer arietinum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.82
pod
wall
Cicer arietinum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.82
1.35
-
-
Cicer arietinum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.82
(S)-malate + NADP+
-
287420
Cicer arietinum
oxaloacetate + NADPH + H+
-
-
-
r
1.1.1.82
oxaloacetate + NADPH
-
287420
Cicer arietinum
(S)-malate + NADP+
-
287420
Cicer arietinum
r
1.1.1.82
oxaloacetate + NADPH
under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis
287420
Cicer arietinum
(S)-malate + NADP+
-
287420
Cicer arietinum
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.82
6
7
-
Cicer arietinum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.82
5.5
8
pH 5.5: about 90% of maximal activity, pH 8.0: about 40% of maximal activity
Cicer arietinum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.82
2-oxoglutarate
5 mM
Cicer arietinum
1.1.1.82
ATP
5 mM
Cicer arietinum
1.1.1.82
citrate
5 mM
Cicer arietinum
1.1.1.82
isocitrate
5 mM
Cicer arietinum
1.1.1.82
malate
5 mM
Cicer arietinum
1.1.1.82
NADP+
5 mM
Cicer arietinum
1.1.1.82
oxalate
5 mM
Cicer arietinum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.82
0.011
-
NADPH
-
Cicer arietinum
1.1.1.82
0.029
-
NADP+
-
Cicer arietinum
1.1.1.82
0.062
-
oxaloacetate
-
Cicer arietinum
1.1.1.82
2
-
(S)-malate
-
Cicer arietinum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.82
135000
-
gel filtration
Cicer arietinum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.82
oxaloacetate + NADPH
Cicer arietinum
under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis
(S)-malate + NADP+
-
Cicer arietinum
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.82
-
Cicer arietinum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.82
pod
wall
Cicer arietinum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.82
1.35
-
-
Cicer arietinum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.82
(S)-malate + NADP+
-
287420
Cicer arietinum
oxaloacetate + NADPH + H+
-
-
-
r
1.1.1.82
oxaloacetate + NADPH
-
287420
Cicer arietinum
(S)-malate + NADP+
-
287420
Cicer arietinum
r
1.1.1.82
oxaloacetate + NADPH
under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis
287420
Cicer arietinum
(S)-malate + NADP+
-
287420
Cicer arietinum
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.82
6
7
-
Cicer arietinum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.82
5.5
8
pH 5.5: about 90% of maximal activity, pH 8.0: about 40% of maximal activity
Cicer arietinum