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Literature summary extracted from

  • Gupta, V.K.; Singh, R.
    Properties of NADP+-malate dehydrogenase from immature pod wall of Cicer arietinum (1990), Plant Physiol. Biochem., 28, 671-678.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.82 2-oxoglutarate 5 mM Cicer arietinum
1.1.1.82 ATP 5 mM Cicer arietinum
1.1.1.82 citrate 5 mM Cicer arietinum
1.1.1.82 isocitrate 5 mM Cicer arietinum
1.1.1.82 malate 5 mM Cicer arietinum
1.1.1.82 NADP+ 5 mM Cicer arietinum
1.1.1.82 oxalate 5 mM Cicer arietinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.82 0.011
-
 NADPH
-
 Cicer arietinum
1.1.1.82 0.029
-
 NADP+
-
 Cicer arietinum
1.1.1.82 0.062
-
 oxaloacetate
-
 Cicer arietinum
1.1.1.82 2
-
 (S)-malate
-
 Cicer arietinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.82 135000
-
 gel filtration Cicer arietinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.82 oxaloacetate + NADPH Cicer arietinum under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis (S)-malate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.82 Cicer arietinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.82
-
 Cicer arietinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.82 pod wall Cicer arietinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.82 1.35
-
-
 Cicer arietinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.82 (S)-malate + NADP+
-
 Cicer arietinum oxaloacetate + NADPH + H+
-
 r
1.1.1.82 oxaloacetate + NADPH
-
 Cicer arietinum (S)-malate + NADP+
-
 r
1.1.1.82 oxaloacetate + NADPH under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis Cicer arietinum (S)-malate + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.82 6 7
-
 Cicer arietinum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.82 5.5 8 pH 5.5: about 90% of maximal activity, pH 8.0: about 40% of maximal activity Cicer arietinum