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Literature summary extracted from

  • Fickenscher, K.; Scheibe, R.
    Limited proteolysis of inactive tetrameric chloroplast NADP-malate dehydrogenase produces active dimers (1988), Arch. Biochem. Biophys., 260, 771-779.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.82 acetone increases the activity of the oxidized truncated enzyme form, optimal concentration 15% Pisum sativum
1.1.1.82 carboxypeptidase Y incubation of the native oxidized enzyme at pH 6.0, 25°C, results in slow activation. A small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding Pisum sativum
1.1.1.82 dithiothreitol activates Pisum sativum
1.1.1.82 methanol increases the activity of the oxidized truncated enzyme form, optimal concentration 8% Pisum sativum

General Stability

EC Number General Stability Organism
1.1.1.82 a small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding Pisum sativum
1.1.1.82 denaturation by 2 M guanidine-HCl Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.82 KCl oxidized truncated enzyme Pisum sativum
1.1.1.82 oxaloacetate pH 8, substrate inhibition of the reduced truncated enzyme Pisum sativum
1.1.1.82 Urea oxidized truncated enzyme Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.82 0.039
-
 NADPH reduced native enzyme Pisum sativum
1.1.1.82 0.042
-
 oxaloacetate reduced truncated enzyme Pisum sativum
1.1.1.82 0.046
-
 NADPH oxidized truncated enzyme Pisum sativum
1.1.1.82 0.048
-
 oxaloacetate reduced native enzyme Pisum sativum
1.1.1.82 0.089
-
 NADPH reduced truncated enzyme Pisum sativum
1.1.1.82 1.2
-
 oxaloacetate oxidized truncated enzyme Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.82 chloroplast
-
 Pisum sativum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.82 38900
-
 4 * 38900, native enzyme, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.82 Pisum sativum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.82 proteolytic modification a small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.82 oxaloacetate + NADPH
-
 Pisum sativum (S)-malate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.82 tetramer 4 * 38900, native enzyme, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.82 6.5 7.5 reduction of oxaloacetate, oxidized truncated enzyme Pisum sativum
1.1.1.82 8 8.5 active enzyme Pisum sativum