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Literature summary extracted from

  • Kleczkowski, L.A.; Edwards, G.E.; Blackwell, R.D.; Lea, P.J.; Givan, C.V.
    Enzymology of the reduction of hydroxypyruvate and glyoxylate in a mutant of barley lacking peroxisomal hydroxypyruvate reductase (1990), Plant Physiol., 94, 819-825.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.81 Hydroxypyruvate 2 mM, 79% inhibition of enzyme activity in LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase Hordeum vulgare
1.1.1.81 Tartronate 2 mM, 83% inhibition of enzyme activity in LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.81 0.08
-
 Hydroxypyruvate enzyme activity of wild-type in 40-60% precipitate fractions after ammonium sulfate fractionation Hordeum vulgare
1.1.1.81 0.71
-
 Hydroxypyruvate enzyme activity of LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase in 40-60% precipitate fractions after ammonium sulfate fractionation Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.81 hydroxypyruvate + NAD(P)H Hordeum vulgare
-
 D-glycerate + NAD(P)+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.81 Hordeum vulgare
-
 barley
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.81 leaf
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.81 0.054
-
 enzyme activity in leaf extracts of LaPr 88/29 mutant which lacks NADH-prefering hydroxypyruvate reductase Hordeum vulgare
1.1.1.81 0.068
-
 enzyme activity in leaf extracts of wild type Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Hordeum vulgare D-glycerate + NAD(P)+ + H+
-
 ?