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Literature summary extracted from

  • Cai, J.; Pietzsch, M.; Theobald, U.; Rizzi, M.
    Fast purification and kinetic studies of the glycerol-3-phosphate dehydrogenase from the yeast Saccharomyces cerevisiae (1996), Biotechnology, 49, 19-27.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 NAD+ competitive inhibitor to NADH at physiological concentration Saccharomyces cerevisiae
1.1.1.8 phosphate competitive inhibitor against dihydroxyacetone phosphate, non-competitive inhibitor against NADH Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Saccharomyces cerevisiae
-
 dihydroxyacetone phosphate + NADH + H+
-
 ?
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Saccharomyces cerevisiae YSH 11-6B
-
 dihydroxyacetone phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Saccharomyces cerevisiae
-
-
-
1.1.1.8 Saccharomyces cerevisiae YSH 11-6B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.8 sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ equilibrium random-bi-bi reaction mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 50.1
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Saccharomyces cerevisiae dihydroxyacetone phosphate + NADH + H+
-
 ?
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Saccharomyces cerevisiae YSH 11-6B dihydroxyacetone phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.8 ? x * 43000-47000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.8 51
-
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 6.8
-
 most active Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Saccharomyces cerevisiae
1.1.1.8 NADH
-
 Saccharomyces cerevisiae