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Literature summary extracted from

  • Kohl, L.; Drmota, T.; Do Thi, C.D.; Callens, M.; Van Beeumen, J.; Opperdoes, F.R.; Michels, P.A.M.
    Clonig and characterization of the NAD-linked glycerol-3-phosphate dehydrogenase of Trypanosoma brucei brucei and Leishmania mexicana mexicana and expression of the trypanosome enzyme in Escherichia coli (1996), Mol. Biochem. Parasitol., 76, 159-173.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.8 recombinant in Escherichia coli Trypanosoma brucei
1.1.1.8 recombinant in Escherichia coli Leishmania mexicana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.8 glycosome
-
 Trypanosoma brucei 20015
-
1.1.1.8 glycosome
-
 Leishmania mexicana 20015
-
1.1.1.8 mitochondrion determined by isopycnic gradient centrifugation Leishmania mexicana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 37651
-
 x * 37651, calculated by cDNA-sequence Trypanosoma brucei
1.1.1.8 39140
-
 x * 39140, calculated by cDNA-sequence Leishmania mexicana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Trypanosoma brucei
-
 dihydroxyacetone phosphate + NADH + H+
-
 ?
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Leishmania mexicana
-
 dihydroxyacetone phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Leishmania mexicana
-
 strain Leishmania mexicana mexicana
-
1.1.1.8 Trypanosoma brucei
-
 strain Trypanosoma brucei brucei
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8
-
 Trypanosoma brucei
1.1.1.8
-
 Leishmania mexicana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 80
-
 estimated Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Trypanosoma brucei dihydroxyacetone phosphate + NADH + H+
-
 ?
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Leishmania mexicana dihydroxyacetone phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.8 ? x * 37651, calculated by cDNA-sequence Trypanosoma brucei
1.1.1.8 ? x * 39140, calculated by cDNA-sequence Leishmania mexicana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Trypanosoma brucei
1.1.1.8 NADH
-
 Trypanosoma brucei