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Literature summary extracted from

  • Marche, S.; Michels, P.A.M.; Opperdoes, F.R.
    Comparative study of Leishmania mexicana and trypanosoma brucei NAD-dependent glycerol-3-phosphate dehydrogenase (2000), Mol. Biochem. Parasitol., 106, 83-91.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.8 recombinant in Escherichia coli Leishmania mexicana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 2-hydroxy-1,2,3-nonadecanetricarboxylic acid trypanocidal drug, IC50 0.00055 mM Leishmania mexicana
1.1.1.8 2-hydroxy-1,2,3-nonadecanetricarboxylic acid IC50 0.0011 mM Trypanosoma brucei
1.1.1.8 dihydroxyacetone phosphate substrate inhibition at 0.2 mM Leishmania mexicana
1.1.1.8 dihydroxyacetone phosphate substrate inhibition at 0.2 mM Trypanosoma brucei
1.1.1.8 Melarsen oxide active principle of the trypanocidal drug melarsoprol and cymelarsen Leishmania mexicana
1.1.1.8 Melarsen oxide active principle of the trypanocidal drug melarsoprol and cymelarsen; cymelarsen, IC50 0.0015-0.005 mM Trypanosoma brucei
1.1.1.8 NaCl strongly inhibits, 50% activity at 250 mM NaCl Leishmania mexicana
1.1.1.8 NaCl strongly inhibits, 50% activity at 250 mM NaCl Trypanosoma brucei
1.1.1.8 NADPH inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate Leishmania mexicana
1.1.1.8 NADPH inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate Trypanosoma brucei
1.1.1.8 Nucleic acids strongly inhibit Leishmania mexicana
1.1.1.8 p-chloromercuribenzoate 0.1 mM, reversed by thiols, e.g. dithiothreitol Trypanosoma brucei
1.1.1.8 suramin trypanocidal drug, potent inhibitor, IC50 0.0002 mM Leishmania mexicana
1.1.1.8 suramin IC50 0.00044 mM Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 0.01
-
 NADH
-
 Trypanosoma brucei
1.1.1.8 0.024
-
 NADH
-
 Leishmania mexicana
1.1.1.8 0.1
-
 dihydroxyacetone phosphate
-
 Trypanosoma brucei
1.1.1.8 0.12
-
 dihydroxyacetone phosphate
-
 Leishmania mexicana
1.1.1.8 0.32
-
 NAD+
-
 Trypanosoma brucei
1.1.1.8 0.42
-
 NAD+
-
 Leishmania mexicana
1.1.1.8 1.7
-
 glycerol-3-phosphate
-
 Leishmania mexicana
1.1.1.8 2.25
-
 glycerol-3-phosphate
-
 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.8 glycosome
-
 Trypanosoma brucei 20015
-
1.1.1.8 glycosome
-
 Leishmania mexicana 20015
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 37000
-
 x * 37000, SDS-PAGE Leishmania mexicana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Trypanosoma brucei
-
 dihydroxyacetone phosphate + NADH + H+
-
 ?
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Leishmania mexicana
-
 dihydroxyacetone phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Leishmania mexicana
-
-
-
1.1.1.8 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8
-
 Trypanosoma brucei
1.1.1.8
-
 Leishmania mexicana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 154
-
-
 Leishmania mexicana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Trypanosoma brucei dihydroxyacetone phosphate + NADH + H+
-
 ?
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Leishmania mexicana dihydroxyacetone phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.8 ? x * 37000, SDS-PAGE Leishmania mexicana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 6.5
-
 glycerol-3-phosphate formation Trypanosoma brucei
1.1.1.8 6.5
-
 glycerol-3-phosphate formation Leishmania mexicana
1.1.1.8 9.6
-
 dihydroxyacetone phosphate formation Trypanosoma brucei
1.1.1.8 9.6
-
 dihydroxyacetone phosphate formation Leishmania mexicana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.8 5 7.5 highest activity, glycerol-3-phosphate formation Trypanosoma brucei
1.1.1.8 5 7.5 highest activity, glycerol-3-phosphate formation Leishmania mexicana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.8 5 9 under diluted conditions Trypanosoma brucei
1.1.1.8 5 9 under diluted conditions Leishmania mexicana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Trypanosoma brucei
1.1.1.8 NADH
-
 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.8 0.0002
-
 trypanocidal drug, potent inhibitor, IC50 0.0002 mM Leishmania mexicana suramin
1.1.1.8 0.00044
-
 IC50 0.00044 mM Trypanosoma brucei suramin
1.1.1.8 0.00055
-
 trypanocidal drug, IC50 0.00055 mM Leishmania mexicana 2-hydroxy-1,2,3-nonadecanetricarboxylic acid
1.1.1.8 0.0011
-
 IC50 0.0011 mM Trypanosoma brucei 2-hydroxy-1,2,3-nonadecanetricarboxylic acid
1.1.1.8 0.0015 0.005 cymelarsen, IC50 0.0015-0.005 mM Trypanosoma brucei Melarsen oxide