EC Number | General Stability | Organism |
---|---|---|
1.1.1.8 | dithiothreitol stabilizes | Drosophila melanogaster |
1.1.1.8 | phenylmethane-sulfonyl fluoride stabilizes during purification | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.8 | ATP | weak inhibitor of both isozymes, i.e. GPDH-1 and -3 | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.8 | 32000 | - |
2 * 32000, SDS-PAGE, confirmed by amino acid composition | Drosophila melanogaster |
1.1.1.8 | 66000 | - |
gel filtration | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Drosophila melanogaster | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.8 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.8 | 3 isozymes in adults and 1 in larvae, distinct in isoelectric focusing | Drosophila melanogaster |
1.1.1.8 | affinity chromatography | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.8 | larva | late third instar | Drosophila melanogaster | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.8 | 156 | - |
isozyme GPDH-3 | Drosophila melanogaster |
1.1.1.8 | 179.8 | - |
isozyme GPDH-1 | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Drosophila melanogaster | dihydroxyacetone phosphate + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.8 | dimer | 2 * 32000, SDS-PAGE, confirmed by amino acid composition | Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.8 | NAD+ | - |
Drosophila melanogaster | |
1.1.1.8 | NADH | - |
Drosophila melanogaster |