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Literature summary extracted from

  • Niesel, D.W.; Bewley, G.C.; Miller, S.G.; Armstrong, F.B.; Lee, C.Y.
    Purification and structural analysis of the soluble sn-glycerol-3-phosphate dehydrogenase isozymes in Drosophila melanogaster (1980), J. Biol. Chem., 255, 4073-4080.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.8 dithiothreitol stabilizes Drosophila melanogaster
1.1.1.8 phenylmethane-sulfonyl fluoride stabilizes during purification Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 ATP weak inhibitor of both isozymes, i.e. GPDH-1 and -3 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 32000
-
2 * 32000, SDS-PAGE, confirmed by amino acid composition Drosophila melanogaster
1.1.1.8 66000
-
gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Drosophila melanogaster
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 3 isozymes in adults and 1 in larvae, distinct in isoelectric focusing Drosophila melanogaster
1.1.1.8 affinity chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.8 larva late third instar Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 156
-
isozyme GPDH-3 Drosophila melanogaster
1.1.1.8 179.8
-
isozyme GPDH-1 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
Drosophila melanogaster dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 dimer 2 * 32000, SDS-PAGE, confirmed by amino acid composition Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
Drosophila melanogaster
1.1.1.8 NADH
-
Drosophila melanogaster