Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Santora, G.T.; Gee, R.; Tolbert, N.E.
    Isolation of a sn-glycerol 3-phosphate: NAD oxidoreductase from spinach leaves (1979), Arch. Biochem. Biophys., 196, 403-411.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.8 freezing inactivates completely, ammonium sulfate prevents inactivation Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 NADH at high concentration Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 0.01
-
NADH
-
Spinacia oleracea
1.1.1.8 0.19
-
NAD+
-
Spinacia oleracea
1.1.1.8 0.3
-
dihydroxyacetone phosphate 0.30-0.35 mM Spinacia oleracea
1.1.1.8 1.6
-
glycerol phosphate
-
Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.8 chloroplast
-
Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 63500
-
gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Spinacia oleracea oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH dihydroxyacetone phosphate + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 affinity chromatography Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.8 leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 37.5
-
-
Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.1.1.8 -20°C, stable as (NH4)2SO4-precipitate Spinacia oleracea
1.1.1.8 4°C, purified and dilute preparation stable for several days in presence of NADH Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 additional information glycerol Spinacia oleracea ?
-
?
1.1.1.8 additional information glyceraldehyde-3-phosphate Spinacia oleracea ?
-
?
1.1.1.8 additional information DL-glyceraldehyde, phosphohydroxypyruvate Spinacia oleracea ?
-
?
1.1.1.8 additional information fructose-6-phosphate, fructose-1,6-bisphosphate Spinacia oleracea ?
-
?
1.1.1.8 additional information no substrates: dihydroxyacetone Spinacia oleracea ?
-
?
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
Spinacia oleracea dihydroxyacetone phosphate + NADH + H+
-
r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH Spinacia oleracea dihydroxyacetone phosphate + NADH + H+
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 6.8
-
reduction of dihydroxyacetone-P Spinacia oleracea
1.1.1.8 9.5
-
oxidation of glycerol-phosphate Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.8 5 8.1 about half-maximal activity at pH 5.0 and 8.1, reduction of dihydroxyacetone phosphate Spinacia oleracea
1.1.1.8 5.8 7.2 reduction of dihydroxyacetone phosphate occurs over a broad range between 5.8 to 7.2 Spinacia oleracea
1.1.1.8 8.6 10 about half-maximal activity at pH 8.6 and 10.0, oxidation of glycerol 3-phosphate Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 additional information no activity with NADPH Spinacia oleracea
1.1.1.8 additional information not NADP+ Spinacia oleracea
1.1.1.8 NAD+
-
Spinacia oleracea
1.1.1.8 NADH
-
Spinacia oleracea