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Literature summary extracted from

  • Collier, G.E.; Sullivan, D.T.; MacIntyre, R.J.
    Purification of alpha-glycerophosphate dehydrogenase from Drosophila melanogaster (1976), Biochim. Biophys. Acta, 429, 316-323.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.8 dithiothreitol stabilizes Drosophila melanogaster
1.1.1.8 EDTA stabilizes Drosophila melanogaster
1.1.1.8 NAD+ stabilizes Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 p-chloromercuribenzoate 0.05 mM, 100% inhibition Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 31700
-
 2 * 31700, SDS-PAGE Drosophila melanogaster
1.1.1.8 67000
-
 gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Drosophila melanogaster
-
 dihydroxyacetone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 2 allelic forms of the enzyme Drosophila melanogaster
1.1.1.8 affinity chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.8 muscle thoracic flight muscle Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 103.5
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Drosophila melanogaster dihydroxyacetone phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 dimer 2 * 31700, SDS-PAGE Drosophila melanogaster

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.8 50
-
 crude extract at least 20 min stable Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Drosophila melanogaster
1.1.1.8 NADH
-
 Drosophila melanogaster