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Literature summary extracted from
- Glycerol phosphate dehydrogenase of chicken breast muscle (1975), Methods Enzymol., 41B, 245-249.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.8 | - |
Gallus gallus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.8 | 2-mercaptoethanol stabilizes during purification | Gallus gallus |
| 1.1.1.8 | ammonium sulfate stabilizes | Gallus gallus |
| 1.1.1.8 | NADH stabilizes preparations of low ionic strength | Gallus gallus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.8 | 0.006 | - |
NADH | - |
Gallus gallus |  |
| 1.1.1.8 | 0.23 | - |
dihydroxyacetone phosphate | - |
Gallus gallus | |
| 1.1.1.8 | 0.5 | - |
NAD+ | - |
Gallus gallus | |
| 1.1.1.8 | 2 | - |
glycerol 3-phosphate | - |
Gallus gallus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Gallus gallus | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.8 | Gallus gallus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.8 | - |
Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.8 | muscle | breast muscle | Gallus gallus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 310 | - |
- |
Gallus gallus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.8 | crystallized, in ammonium sulfate stable for months | Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Gallus gallus | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 7.5 | 8 | pH-rate profile for the reduction of dihydroxyacetone phosphate shows rather sharp optimum between pH 7.5 and 8.0 | Gallus gallus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | additional information | no activity with NADPH | Gallus gallus | |
| 1.1.1.8 | NAD+ | - |
Gallus gallus | |
| 1.1.1.8 | NADH | - |
Gallus gallus | |
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Release 2023.1 (January 2023)
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