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Literature summary extracted from
- alpha-Glycerophosphate dehydrogenase (1963), The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 7, 85-96.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | EDTA | without EDTA the rates are about 60% of the maximal rate | Oryctolagus cuniculus |  |
| 1.1.1.8 | glycine buffer | 0.9 M, slight activation | Oryctolagus cuniculus | |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.8 | - |
Oryctolagus cuniculus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.8 | charcoal inactivates rabbit enzyme, restorable by thiamic acid | Oryctolagus cuniculus |
| 1.1.1.8 | EDTA stabilizes | Oryctolagus cuniculus |
| 1.1.1.8 | even cold dilute solutions in 0.2 M ammonium sulfate at pH 5.8 are stable for weeks | Oryctolagus cuniculus |
| 1.1.1.8 | redistilled water prevents denaturation in absence of salts | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | 2,3-Dimercaptopropanol | competitive inhibitor to glycerophosphate | Oryctolagus cuniculus | |
| 1.1.1.8 | iodoacetate | - |
Homo sapiens | |
| 1.1.1.8 | iodoacetate | 0.1 mM 23% inhibition | Oryctolagus cuniculus | |
| 1.1.1.8 | iodoacetate | - |
Rattus norvegicus | |
| 1.1.1.8 | additional information | coenzyme analogs: acetylpyridine-NAD+, deaminoacetylpyridine-NAD+, pyridinealdehyde-NAD+, deaminopyridinealdehyde-NAD+, potent inhibitors | Oryctolagus cuniculus | |
| 1.1.1.8 | N-ethylmaleimide | 0.1 mM complete inhibition | Oryctolagus cuniculus | |
| 1.1.1.8 | N-ethylmaleimide | - |
Rattus norvegicus | |
| 1.1.1.8 | p-mercuribenzoate | 0.1 mM complete inhibition | Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.8 | 0.11 | - |
alpha-glycerophosphate | - |
Oryctolagus cuniculus | |
| 1.1.1.8 | 0.38 | - |
NAD+ | - |
Oryctolagus cuniculus | |
| 1.1.1.8 | 0.46 | - |
dihydroxyacetone phosphate | - |
Oryctolagus cuniculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.8 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
| 1.1.1.8 | cytoplasm | - |
Oryctolagus cuniculus | 5737 | - |
| 1.1.1.8 | cytoplasm | - |
Locusta migratoria | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 78000 | - |
- |
Rattus norvegicus |
| 1.1.1.8 | 78000 | - |
calculated from partial specific volume, sedimentation and diffusion data | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Homo sapiens | equilibrium far to the side of alpha-glycerophosphate at neutral pH | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Rattus norvegicus | equilibrium far to the side of alpha-glycerophosphate at neutral pH | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Oryctolagus cuniculus | equilibrium far to the side of alpha-glycerophosphate at neutral pH | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.8 | Bos taurus | - |
- |
- |
| 1.1.1.8 | Homo sapiens | - |
- |
- |
| 1.1.1.8 | Locusta migratoria | - |
- |
- |
| 1.1.1.8 | Mus musculus | - |
- |
- |
| 1.1.1.8 | Oryctolagus cuniculus | - |
- |
- |
| 1.1.1.8 | Rattus norvegicus | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | one active site per enzyme molecule | Homo sapiens | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | one active site per enzyme molecule | Rattus norvegicus | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | one active site per enzyme molecule | Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.8 | breast cancer cell | - |
Homo sapiens | - |
| 1.1.1.8 | kidney | - |
Homo sapiens | - |
| 1.1.1.8 | kidney | - |
Rattus norvegicus | - |
| 1.1.1.8 | leukocyte | also other blood components | Homo sapiens | - |
| 1.1.1.8 | leukocyte | also other blood components | Rattus norvegicus | - |
| 1.1.1.8 | liver | - |
Homo sapiens | - |
| 1.1.1.8 | liver | - |
Rattus norvegicus | - |
| 1.1.1.8 | liver | - |
Oryctolagus cuniculus | - |
| 1.1.1.8 | muscle | - |
Mus musculus | - |
| 1.1.1.8 | muscle | - |
Rattus norvegicus | - |
| 1.1.1.8 | muscle | - |
Oryctolagus cuniculus | - |
| 1.1.1.8 | muscle | bovine smooth muscles | Bos taurus | - |
| 1.1.1.8 | sarcoma cell | - |
Homo sapiens | - |
| 1.1.1.8 | sarcoma cell | - |
Oryctolagus cuniculus | - |
| 1.1.1.8 | sarcoma cell | Crocker sarcoma | Mus musculus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.8 | 4°C, dilute solution, pH 5.8, stable for weeks | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | additional information | - |
Oryctolagus cuniculus | ? | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Homo sapiens | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Rattus norvegicus | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Oryctolagus cuniculus | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | equilibrium far to the side of alpha-glycerophosphate at neutral pH | Homo sapiens | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | equilibrium far to the side of alpha-glycerophosphate at neutral pH | Rattus norvegicus | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | equilibrium far to the side of alpha-glycerophosphate at neutral pH | Oryctolagus cuniculus | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 20 | - |
stable for at least 30 min | Oryctolagus cuniculus |
| 1.1.1.8 | 55 | - |
t1/2: 1 min | Oryctolagus cuniculus |
| 1.1.1.8 | 60 | - |
- |
Mus musculus |
| 1.1.1.8 | 60 | - |
- |
Homo sapiens |
| 1.1.1.8 | 60 | - |
- |
Rattus norvegicus |
| 1.1.1.8 | 60 | - |
- |
Bos taurus |
| 1.1.1.8 | 60 | - |
complete inactivation after 1 min | Oryctolagus cuniculus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.8 | 345 | - |
dihydroxyacetone phosphate | reduction of synthetic dihydroxyacetone phosphate, 20°C, pH 7, rabbit muscle | Oryctolagus cuniculus | |
| 1.1.1.8 | 390 | - |
dihydroxyacetone phosphate | reduction of synthetic dihydroxyacetone phosphate, 20°C, pH 7, rabbit muscle, under conditions where competitive inhibition by fructose 1,6-diphosphate are not excluded | Oryctolagus cuniculus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 7.5 | - |
reduction of dihydroxyacetone phosphate, in 0.2 M phosphate buffer | Oryctolagus cuniculus |
| 1.1.1.8 | 10.2 | - |
oxidation of glycerol 3-phosphate, in 0.03 glycine buffer | Oryctolagus cuniculus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 5.7 | 8.5 | around this range, most stable | Oryctolagus cuniculus |
| 1.1.1.8 | 7 | - |
less stable | Oryctolagus cuniculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | additional information | - |
Homo sapiens | |
| 1.1.1.8 | additional information | deamino-NAD+ 60.3% as effective as NAD+ | Oryctolagus cuniculus | |
| 1.1.1.8 | NAD+ | - |
Mus musculus | |
| 1.1.1.8 | NAD+ | - |
Homo sapiens | |
| 1.1.1.8 | NAD+ | - |
Rattus norvegicus | |
| 1.1.1.8 | NAD+ | - |
Bos taurus | |
| 1.1.1.8 | NAD+ | - |
Oryctolagus cuniculus | |
| 1.1.1.8 | NADH | - |
Mus musculus | |
| 1.1.1.8 | NADH | - |
Bos taurus | |
| 1.1.1.8 | NADH | 1 mol per mol enzyme | Homo sapiens | |
| 1.1.1.8 | NADH | 1 mol per mol enzyme | Rattus norvegicus | |
| 1.1.1.8 | NADH | 1 mol per mol enzyme | Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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