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Literature summary extracted from

  • Albertyn, J.; Van Tonder, A.; Prior, B.A.
    Purification and characterization of glycerol-3-phosphate dehydrogenase of Saccharomyces cerevisiae (1992), FEBS Lett., 308, 130-132.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 ADP at physiological concentration, 10 mM 90% inhibition Saccharomyces cerevisiae
1.1.1.8 ATP at physiological concentration, 10 mM 95% inhibition Saccharomyces cerevisiae
1.1.1.8 fructose-1,6-bisphosphate at physiological concentration, non-competitive Saccharomyces cerevisiae
1.1.1.8 additional information no inhibition by NADPH, acetaldehyde, glycerol, ethanol Saccharomyces cerevisiae
1.1.1.8 NAD+ competitive inhibitor to NADH at physiological concentration Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 0.023
-
 NADH
-
 Saccharomyces cerevisiae
1.1.1.8 0.54
-
 dihydroxyacetone phosphate
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 42000
-
 2 * 42000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.8 68000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Saccharomyces cerevisiae oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Saccharomyces cerevisiae H44-3D oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 dihydroxyacetone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Saccharomyces cerevisiae
-
-
-
1.1.1.8 Saccharomyces cerevisiae H44-3D
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 41.1
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.1.8 -20°C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable Saccharomyces cerevisiae
1.1.1.8 -80°C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable Saccharomyces cerevisiae
1.1.1.8 4°C, 1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, eluted from Sephadex G-100 column, 90% activity remained after several weeks Saccharomyces cerevisiae
1.1.1.8 4°C, stable for several weeks in 1 M Tris/HCl buffer with 2-mercaptoethanol, pH 7.0 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 additional information glycerol Saccharomyces cerevisiae ?
-
 ?
1.1.1.8 additional information no substrates: dihydroxyacetone Saccharomyces cerevisiae ?
-
 ?
1.1.1.8 additional information glycerol Saccharomyces cerevisiae H44-3D ?
-
 ?
1.1.1.8 additional information no substrates: dihydroxyacetone Saccharomyces cerevisiae H44-3D ?
-
 ?
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Saccharomyces cerevisiae dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 Saccharomyces cerevisiae dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Saccharomyces cerevisiae H44-3D dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 Saccharomyces cerevisiae H44-3D dihydroxyacetone phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 dimer 2 * 42000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 7.6
-
 maximum rate of dihydroxyacetone phosphate reduction Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.8 7 8 dihydroxyacetone reduction, activity decreases rapidly below pH 7.0 and above pH 8.0, more rapidly at acidic values Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 additional information no activity with NADPH Saccharomyces cerevisiae
1.1.1.8 NAD+
-
 Saccharomyces cerevisiae
1.1.1.8 NADH
-
 Saccharomyces cerevisiae