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Literature summary extracted from

  • Tochikura, T.; Fukuda, H.; Moriguchi, M.
    Purification and properties of glyoxylate reductase I from baker's yeast (1979), J. Biochem., 86, 105-110.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.79 2-mercaptoethanol isoenzyme 1, 1 mM, 19% inhibition Saccharomyces cerevisiae
1.1.1.79 cysteine isoenzyme 1, 1 mM, 29% inhibition Saccharomyces cerevisiae
1.1.1.79 dithiothreitol isoenzyme 1, 1 mM, 41% inhibition, 10 mM, 87% inhibition Saccharomyces cerevisiae
1.1.1.79 glutathione isoenzyme 1, 1 mM, 12% inhibition Saccharomyces cerevisiae
1.1.1.79 iodoacetamide isoenzyme 2, 1 mM, 87% inhibition, isoenzyme 1, 1 mM, 17% inhibition Saccharomyces cerevisiae
1.1.1.79 iodoacetate isoenzyme 2, 1 mM, 97% inhibition, isoenzyme 1, 1 mM, 18% inhibition Saccharomyces cerevisiae
1.1.1.79 nitrate isoenzyme 1, 50 mM, 50% inhibition Saccharomyces cerevisiae
1.1.1.79 p-chloromercuribenzoate isoenzyme 2, 1 mM, complete inhibition, 0.1 mM, 88% inhibition, 29% inhibition in the presence of 1 mM dithiothreitol, 21% inhibition in the presence of 1 mM L-cysteine, isoenzyme 1, 1 mM, 59% inhibition Saccharomyces cerevisiae
1.1.1.79 Sodium fluoride isoenzyme 1, 10 mM, 32% inhibition Saccharomyces cerevisiae
1.1.1.79 sodium iodide isoenzyme 1, 50 mM, 37% inhibition of glyoxylate reduction Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.004
-
 NADPH isoenzyme 1 Saccharomyces cerevisiae
1.1.1.79 13
-
 glyoxylate isoenzyme 1 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.79 31000
-
 isoenzyme 1, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H Saccharomyces cerevisiae
-
 glycolate + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 isoenzyme 1, protamine sulfate, DEAE-cellulose, hydroxylapatite, Sephadex G-150, DEAE-cellulose, hydroxylapatite Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.79 3.89
-
 isoenzyme 1 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.1.79 isoenzyme 1, 4°C, 10 mM potassium phosphate, pH 7.0, 1 week, 20% loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxal + NADPH isoenzyme 1, 16% activity of glyxoxylate Saccharomyces cerevisiae glycol + NADP+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H
-
 Saccharomyces cerevisiae glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H
-
 Saccharomyces cerevisiae glycolate + NAD(P)+
-
 ir
1.1.1.79 hydroxypyruvate + NAD(P)H + H+ isoenzyme 1, hydroxypyruvate shows 15% of the activity of glyoxylate Saccharomyces cerevisiae glycerate + NAD(P)+
-
 ?
1.1.1.79 oxaloacetate + NADPH isoenzyme 1, 12% activity of glyoxylate Saccharomyces cerevisiae malate + NADP+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.79 40
-
 isoenzyme 1, 20% loss of activity after 5 min, complete inactivation above 50°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.79 5.5 7.2 isoenzyme 1 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NAD(P)H specific for NADPH Saccharomyces cerevisiae
1.1.1.79 NADPH specific for NADPH Saccharomyces cerevisiae