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Literature summary extracted from

  • Willetts, A.J.; Turner, J.M.
    Threonine metabolism in a strain of Bacillus subtilis enzymic oxidation of 1-aminopropan-2-ol and aminoacetone (1971), Biochim. Biophys. Acta, 252, 98-104.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.75 31
-
 L-1-Aminopropan-2-ol
-
 Bacillus subtilis
1.1.1.75 54
-
 D-1-aminopropan-2-ol
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.75 K+ stimulating at all concentrations Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.75 250000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.75 L-1-aminopropan-2-ol + NAD+ Bacillus subtilis
-
 aminoacetone + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.75 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.75 partial Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.75 0.001
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
1.1.1.75 -15°C, 0.1 M Tris/HCl buffer, pH 7.2, 50% loss of activity Bacillus subtilis
1.1.1.75 0°C, 0.1 M Tris/HCl buffer, pH 7.2, 90% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.75 1,3-diaminopropan-2-ol + NAD+
-
 Bacillus subtilis 2-aminoacetamide + NADH
-
 ?
1.1.1.75 1-amino-3-diethylaminopropan-2-ol + NAD+
-
 Bacillus subtilis 2-amino-N,N-diethylacetamide + NADH
-
 ?
1.1.1.75 1-aminopropan-2,3-diol + NAD+
-
 Bacillus subtilis ?
-
 ?
1.1.1.75 butan-2,3-diol + NAD+
-
 Bacillus subtilis ?
-
 ?
1.1.1.75 D,L-1-aminopropan-2-ol + NAD+ D-enantiomer 50% of activity with L-enantiomer Bacillus subtilis aminoacetone + NADH
-
 ir
1.1.1.75 D-1-aminopropan-2-ol + NAD+
-
 Bacillus subtilis aminoacetone + NADH + H+
-
 r
1.1.1.75 hydroxyacetone + NAD+ no activity with acetone Bacillus subtilis 2-oxopropanal + NADH
-
 ?
1.1.1.75 L-1-aminopropan-2-ol + NAD+
-
 Bacillus subtilis aminoacetone + NADH
-
 ir
1.1.1.75 propan-1,2-diol + NAD+
-
 Bacillus subtilis ?
-
 ?
1.1.1.75 propan-1,3-diol + NAD+
-
 Bacillus subtilis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.75 45
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.75 20 60
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.75 8.5
-
 sharp optimum Bacillus subtilis