BRENDA - Enzyme Database

The enzymatic reduction of retinal to retinol in rat intestine

Fidge, N.H.; Goodman, D.S.; J. Biol. Chem. 243, 4372-4379 (1968)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.71
cysteine
-
Rattus norvegicus
1.1.1.71
GSH
-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.71
2-mercaptoethanol
slight inhibition
Rattus norvegicus
1.1.1.71
N-ethylmaleimide
strong inhibition
Rattus norvegicus
1.1.1.71
p-hydroxymercuribenzoate
strong inhibition
Rattus norvegicus
1.1.1.71
Sodium glycocholate
-
Rattus norvegicus
1.1.1.71
Tween 80
-
Rattus norvegicus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.71
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.71
ammonium sulfate, DEAE-Sephadex, Sephadex G-200, 55°C for 6 min
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.71
benzaldehyde + NAD(P)H
-
287282
Rattus norvegicus
benzyl alcohol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
butanal + NAD(P)H + H+
-
287282
Rattus norvegicus
butan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
citral + NAD(P)H
-
287282
Rattus norvegicus
citrol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
decanal + NAD(P)H
-
287282
Rattus norvegicus
decan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
dodecanal + NAD(P)H
-
287282
Rattus norvegicus
dodecan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
ethanal + NAD(P)H
-
287282
Rattus norvegicus
ethanol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
farnesyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
farnesol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
heptanal + NAD(P)H
-
287282
Rattus norvegicus
heptan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
linolenyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
linolenol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
linoleyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
linoleol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
octanal + NAD(P)H + H+
-
287282
Rattus norvegicus
octan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
oleyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
oleol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
retinal + NAD(P)H
-
287282
Rattus norvegicus
retinol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
tetradecanal + NAD(P)H
-
287282
Rattus norvegicus
tetradecan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.71
6.3
-
reduction of retinal, sharp decrease above pH 6.3 and below pH 6.0
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.71
5.5
7
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.71
NAD(P)H
half maximal velocity with 0.0004 mM NADH and 0.04 mM NADPH
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.71
cysteine
-
Rattus norvegicus
1.1.1.71
GSH
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.71
NAD(P)H
half maximal velocity with 0.0004 mM NADH and 0.04 mM NADPH
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.71
2-mercaptoethanol
slight inhibition
Rattus norvegicus
1.1.1.71
N-ethylmaleimide
strong inhibition
Rattus norvegicus
1.1.1.71
p-hydroxymercuribenzoate
strong inhibition
Rattus norvegicus
1.1.1.71
Sodium glycocholate
-
Rattus norvegicus
1.1.1.71
Tween 80
-
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.71
ammonium sulfate, DEAE-Sephadex, Sephadex G-200, 55°C for 6 min
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.71
benzaldehyde + NAD(P)H
-
287282
Rattus norvegicus
benzyl alcohol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
butanal + NAD(P)H + H+
-
287282
Rattus norvegicus
butan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
citral + NAD(P)H
-
287282
Rattus norvegicus
citrol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
decanal + NAD(P)H
-
287282
Rattus norvegicus
decan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
dodecanal + NAD(P)H
-
287282
Rattus norvegicus
dodecan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
ethanal + NAD(P)H
-
287282
Rattus norvegicus
ethanol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
farnesyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
farnesol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
heptanal + NAD(P)H
-
287282
Rattus norvegicus
heptan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
linolenyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
linolenol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
linoleyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
linoleol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
octanal + NAD(P)H + H+
-
287282
Rattus norvegicus
octan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
oleyl aldehyde + NAD(P)H
-
287282
Rattus norvegicus
oleol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
retinal + NAD(P)H
-
287282
Rattus norvegicus
retinol + NAD(P)+
-
287282
Rattus norvegicus
?
1.1.1.71
tetradecanal + NAD(P)H
-
287282
Rattus norvegicus
tetradecan-1-ol + NAD(P)+
-
287282
Rattus norvegicus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.71
6.3
-
reduction of retinal, sharp decrease above pH 6.3 and below pH 6.0
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.71
5.5
7
-
Rattus norvegicus