Literature summary extracted from

Schafer, A.; Stein, M.A.; Schneider, K.H.; Giffhorn, F.
Mannitol dehydrogenase from Rhodobacter sphaeroides Si4: subcloning, overexpression in Escherichia coli and characterization of the recombinant enzyme (1997), Appl. Microbiol. Biotechnol., 48, 47-52.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.67	expression in Escherichia coli	Cereibacter sphaeroides

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.67	0.6	-	D-mannitol	recombinant protein	Cereibacter sphaeroides
1.1.1.67	1.6	-	D-arabitol	recombinant protein	Cereibacter sphaeroides
1.1.1.67	58.5	-	D-sorbitol	recombinant protein	Cereibacter sphaeroides

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.67	54000	-	recombinant protein, SDS-PAGE	Cereibacter sphaeroides

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.67	Cereibacter sphaeroides	-	phototrophic bacterium	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.67	recombinant protein	Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.67	68.3	-	recombinant protein	Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.67	D-arabitol + NAD+	-	Cereibacter sphaeroides	? + NADH	-	?
1.1.1.67	D-glucitol + NAD+	-	Cereibacter sphaeroides	? + NADH	-	?
1.1.1.67	D-mannitol + NAD+	-	Cereibacter sphaeroides	D-fructose + NADH + H+	-	?
1.1.1.67	D-sorbitol + NAD+	-	Cereibacter sphaeroides	?	-	r
1.1.1.67	L-sorbitol + NAD+	-	Cereibacter sphaeroides	L-sorbose + NADH + H+	-	?

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Release 2023.1 (January 2023)