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Literature summary extracted from
- Mannitol dehydrogenase (crystalline) from Lactobacillus brevis (1966), Methods Enzymol., 9, 143-146.
No PubMed abstract available
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | - |
Levilactobacillus brevis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.67 | D-mannitol + NAD+ | Levilactobacillus brevis | - |
D-fructose + NADH + H+ | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.67 | Levilactobacillus brevis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | - |
Levilactobacillus brevis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 27.3 | - |
- |
Levilactobacillus brevis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.67 | -16°C, 0.02 M sodium acetate buffer pH 6.0, 1 mM 2-mercaptoethanol, several weeks | Levilactobacillus brevis |
| 1.1.1.67 | 3°C, crystalline enzyme in ammonium sulfate, 2 months | Levilactobacillus brevis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.67 | D-mannitol + NAD+ | - |
Levilactobacillus brevis | D-fructose + NADH + H+ | - |
? | |
| 1.1.1.67 | D-mannitol + NAD+ | enzyme highly specific for D-mannitol and D-fructose | Levilactobacillus brevis | D-fructose + NADH + H+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 5.3 | - |
D-fructose reduction | Levilactobacillus brevis |
| 1.1.1.67 | 8.6 | - |
D-mannitol oxidation | Levilactobacillus brevis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 6 | 6.5 | - |
Levilactobacillus brevis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.67 | NAD+ | - |
Levilactobacillus brevis | |
| 1.1.1.67 | NADH | - |
Levilactobacillus brevis | |
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Release 2023.1 (January 2023)
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