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Literature summary extracted from
- Purification, molecular cloning, and catalytic activity of Schizosaccharomyces pombe pyridoxal reductase (1999), J. Biol. Chem., 274, 23185-23190.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.65 | expression in Escherichia coli | Schizosaccharomyces pombe |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.65 | purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40 | Schizosaccharomyces pombe |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.65 | 0.096 | - |
2-nitrobenzaldehyde | - |
Schizosaccharomyces pombe |  |
| 1.1.1.65 | 0.9 | - |
pyridoxal | - |
Schizosaccharomyces pombe | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.65 | 41000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.65 | pyridoxine + NADP+ | Schizosaccharomyces pombe | - |
pyridoxal + NADPH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.65 | Schizosaccharomyces pombe | O14295 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.65 | - |
Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.65 | 2-nitrobenzaldehyde + NADPH | - |
Schizosaccharomyces pombe | 2-nitrobenzyl alcohol + NADP+ | - |
? | |
| 1.1.1.65 | pyridoxine + NADP+ | - |
Schizosaccharomyces pombe | pyridoxal + NADPH | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.65 | 6.5 | 7.5 | for formation of pyridoxine | Schizosaccharomyces pombe |
| 1.1.1.65 | 7.5 | 8.5 | for formation of pyridoxal | Schizosaccharomyces pombe |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.65 | NADP+ | - |
Schizosaccharomyces pombe | |
| 1.1.1.65 | NADPH | - |
Schizosaccharomyces pombe | |
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Release 2023.1 (January 2023)
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