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Literature summary extracted from

  • Renwick, A.G.C.; Soon, C.Y.; Chambers, S.M.; Brown, C.R.
    Estradiol-17beta dehydrogenase from chicken liver (1981), J. Biol. Chem., 256, 1881-1887.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.62 16alpha-Bromoacetoxyprogesterone
-
 Gallus gallus
1.1.1.62 PCMB 0.1 mM, complete loss of activity Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.62 0.0077
-
 NADP+
-
 Gallus gallus
1.1.1.62 0.0173
-
 estradiol-17beta in absence of bovine serum albumin Gallus gallus
1.1.1.62 0.025
-
 estradiol-17beta in presence of bovine serum albumin Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.62 43000
-
 and a second enzyme form of 97000 Da, gel filtration Gallus gallus
1.1.1.62 97000
-
 and a second enzyme form of 97000 Da Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.62 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.62
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.62 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.62 289
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
1.1.1.62 -20°C, 50% v/v glycerol, stable for at least 3 months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.62 epitestosterone + NADP+ poor substrate Gallus gallus 4-androsten-3,17-dione + NADPH + H+
-
 ?
1.1.1.62 estradiol-17alpha + NADP+ at about 3% of the activity with estradiol-17beta Gallus gallus estrone + NADPH
-
 ?
1.1.1.62 estradiol-17beta + NADP+ rate of oxidation of estradiol-17beta at the optimum, pH 9.9, is three to four times faster than that of the reverse reaction at pH 7.4 Gallus gallus estrone + NADPH
-
 r
1.1.1.62 estrone + NADPH + H+ rate of oxidation of estradiol-17beta at the optimum, pH 9.9, is three to four times faster than that of the reverse reaction at pH 7.4 Gallus gallus estradiol-17beta + NADP+
-
 r
1.1.1.62 testosterone + NADP+ poor substrate Gallus gallus 4-androstene-3,17-dione + NADPH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.62 9.9
-
 NADP+-linked oxidation of estradiol-17beta Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.62 NADP+ cofactor Gallus gallus
1.1.1.62 NADPH cofactor Gallus gallus