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Literature summary extracted from

  • Wolff, R.A.; Kenealy W.R.
    Purification and characterization of the oxygen-sensitive 4-hydroxybutanoate dehydrogenase from Clostridium kluyveri (1995), Protein Expr. Purif., 6, 206-212.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.61 oxygen
-
 Clostridium kluyveri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.61 0.055
-
 4-hydroxybutanoate
-
 Clostridium kluyveri
1.1.1.61 0.15
-
 NADH
-
 Clostridium kluyveri
1.1.1.61 0.56
-
 succinate semialdehyde
-
 Clostridium kluyveri
1.1.1.61 0.67
-
 NAD+
-
 Clostridium kluyveri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.61 Cu monomeric units contains two atoms Clostridium kluyveri
1.1.1.61 Fe monomeric unit contains one atom Clostridium kluyveri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.61 41600
-
 2 * 41600, SDS-PAGE Clostridium kluyveri
1.1.1.61 86000
-
 gel filtration Clostridium kluyveri

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.61 Clostridium kluyveri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.61
-
 Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.61 4-hydroxybutanoate + NAD+
-
 Clostridium kluyveri succinate semialdehyde + NADH + H+
-
 r
1.1.1.61 succinate semialdehyde + NADH + H+
-
 Clostridium kluyveri 4-hydroxybutanoate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.61 dimer 2 * 41600, SDS-PAGE Clostridium kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.61 6.1
-
 reduction of 4-hydroxybutanoate Clostridium kluyveri
1.1.1.61 9.4
-
 oxidation of 4-hydroxybutanoate Clostridium kluyveri