BRENDA - Enzyme Database

Tartaric acid metabolism. 8. Crystalline tartronic semialdehyde reductase

Kohn, L.; J. Biol. Chem. 243, 4426-4433 (1968)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.60
-
Pseudomonas putida
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.60
beta-Mercaptopyruvate
-
Pseudomonas putida
1.1.1.60
Dihydroxyfumarate
-
Pseudomonas putida
1.1.1.60
glycolate
-
Pseudomonas putida
1.1.1.60
Malonic semialdehyde
-
Pseudomonas putida
1.1.1.60
Succinic semialdehyde
-
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.60
0.02
0.027
NADH
-
Pseudomonas putida
1.1.1.60
0.036
0.05
NADPH
-
Pseudomonas putida
1.1.1.60
0.04
-
NAD+
-
Pseudomonas putida
1.1.1.60
0.05
0.4
D-glycerate
-
Pseudomonas putida
1.1.1.60
0.05
0.4
tartronate semialdehyde
-
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.60
26000
-
4 * 26000, sedimentation velocity after treatment with guanidine hydrochloride and mercaptoethanol, amino acid analysis
Pseudomonas putida
1.1.1.60
104000
-
sedimentation velocity
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.60
tartronate semialdehyde + NAD(P)H
Pseudomonas putida
-
(R)-glycerate + NAD(P)+
-
Pseudomonas putida
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.60
Pseudomonas putida
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.60
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.60
50
-
-
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.60
hydroxypyruvate + NAD(P)H + H+
-
287164
Pseudomonas putida
glycerate + NAD(P)+
-
287164
Pseudomonas putida
r
1.1.1.60
tartronate semialdehyde + NAD(P)H
-
287164
Pseudomonas putida
(R)-glycerate + NAD(P)+
-
287164
Pseudomonas putida
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.60
tetramer
4 * 26000, sedimentation velocity after treatment with guanidine hydrochloride and mercaptoethanol, amino acid analysis
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.60
6
-
-
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.60
NAD+
-
Pseudomonas putida
1.1.1.60
NADH
-
Pseudomonas putida
1.1.1.60
NADP+
-
Pseudomonas putida
1.1.1.60
NADPH
-
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.60
NAD+
-
Pseudomonas putida
1.1.1.60
NADH
-
Pseudomonas putida
1.1.1.60
NADP+
-
Pseudomonas putida
1.1.1.60
NADPH
-
Pseudomonas putida
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.60
-
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.60
beta-Mercaptopyruvate
-
Pseudomonas putida
1.1.1.60
Dihydroxyfumarate
-
Pseudomonas putida
1.1.1.60
glycolate
-
Pseudomonas putida
1.1.1.60
Malonic semialdehyde
-
Pseudomonas putida
1.1.1.60
Succinic semialdehyde
-
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.60
0.02
0.027
NADH
-
Pseudomonas putida
1.1.1.60
0.036
0.05
NADPH
-
Pseudomonas putida
1.1.1.60
0.04
-
NAD+
-
Pseudomonas putida
1.1.1.60
0.05
0.4
D-glycerate
-
Pseudomonas putida
1.1.1.60
0.05
0.4
tartronate semialdehyde
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.60
26000
-
4 * 26000, sedimentation velocity after treatment with guanidine hydrochloride and mercaptoethanol, amino acid analysis
Pseudomonas putida
1.1.1.60
104000
-
sedimentation velocity
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.60
tartronate semialdehyde + NAD(P)H
Pseudomonas putida
-
(R)-glycerate + NAD(P)+
-
Pseudomonas putida
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.60
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.60
50
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.60
hydroxypyruvate + NAD(P)H + H+
-
287164
Pseudomonas putida
glycerate + NAD(P)+
-
287164
Pseudomonas putida
r
1.1.1.60
tartronate semialdehyde + NAD(P)H
-
287164
Pseudomonas putida
(R)-glycerate + NAD(P)+
-
287164
Pseudomonas putida
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.60
tetramer
4 * 26000, sedimentation velocity after treatment with guanidine hydrochloride and mercaptoethanol, amino acid analysis
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.60
6
-
-
Pseudomonas putida