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Literature summary extracted from

  • Burleigh, B.D.; Rigby, P.W.J.; Hartley, B.S.
    A comparison of wild-type and mutant ribitol dehydrogenases from Klebsiella aerogenes (1974), Biochem. J., 143, 341-352.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.56 freezing and thawing causes 30-40% decrease of mutant B enzyme activity Klebsiella aerogenes
1.1.1.56 freezing and thawing causes 5-8% decrease of wild type enzyme activity Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.56 8
-
 ribitol pH 7 Klebsiella aerogenes
1.1.1.56 147
-
 L-arabitol pH 7 Klebsiella aerogenes
1.1.1.56 579
-
 xylitol pH 7 Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.56 25000
-
 4 * 25000, wild type enzyme, SDS-PAGE Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.56 ribitol + NAD+ Klebsiella aerogenes
-
 D-ribulose + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.56 Klebsiella aerogenes
-
 inducible enzyme
-
1.1.1.56 Klebsiella aerogenes
-
 mutants with constitutive enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.56 ribitol dehydrogenase B Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.56 43.6
-
 ribitol dehydrogenase B Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.56 mutant B enzyme inactive after freezing for 2 months, half life of 10 days at 2°C Klebsiella aerogenes
1.1.1.56 wild type enzyme can be stored frozen for months without greater loss of activity , half life of 70 days at 2°C Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.56 L-arabitol + NAD+
-
 Klebsiella aerogenes L-xylulose + NADH + H+
-
 ?
1.1.1.56 ribitol + NAD+
-
 Klebsiella aerogenes D-ribulose + NADH
-
 ?
1.1.1.56 xylitol + NAD+ mutants have increased xylitol activity Klebsiella aerogenes D-xylulose + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.56 ? monomers of 27000, mutant B, SDS-PAGE Klebsiella aerogenes
1.1.1.56 tetramer 4 * 25000, wild type enzyme, SDS-PAGE Klebsiella aerogenes

Synonyms

EC Number Synonyms Comment Organism
1.1.1.56 ribitol dehydrogenase B mutant enzyme with different properties Klebsiella aerogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.56 28
-
 L-arabitol
-
 Klebsiella aerogenes
1.1.1.56 36
-
 xylitol
-
 Klebsiella aerogenes
1.1.1.56 299
-
 ribitol
-
 Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.56 NAD+
-
 Klebsiella aerogenes
1.1.1.56 NADH
-
 Klebsiella aerogenes