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Literature summary extracted from

  • Kazeto, Y.; Adachi, S.; Yamauchi, K.
    20beta-hydroxysteroid dehydrogenase of the japanes eel ovary: its cellular localization and changes in the enzymatic acitvity during sexual maturation (2001), Gen. Comp. Endocrinol., 122, 109-115.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.53 salmon gonadotropin stimulates the membrane bound part of the enzyme Anguilla japonica

General Stability

EC Number General Stability Organism
1.1.1.53 presence of octyl glucoside, 40.5% of enzyme activity from sonicated mitochondria retained, 20% glycerol Anguilla japonica
1.1.1.53 several detergents tested for solubilization Anguilla japonica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.53 microsome
-
 Anguilla japonica
-
-
1.1.1.53 mitochondrial membrane
-
 Anguilla japonica 31966
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ Anguilla japonica
-
 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.53 deoxycholate 40.1% of enzyme activity from sonicated mitochondria retained, 20% glycerol Anguilla japonica

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.53 Anguilla japonica
-
 japanese eel
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.53 ovary
-
 Anguilla japonica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.53 additional information
-
 specific activity about 3.8 pmol/min/mg in ovulated oocyte microsomes and 6.9 pmol/min/mg in ovulated oocyte mitochondria, 11.2 pmol/min/mg in ovarian tissue mitochondria after ovulation Anguilla japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+
-
 Anguilla japonica 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+
-
 ?
1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+
-
 Anguilla japonica 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ?