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Literature summary extracted from

  • Tyrakowska, B.; Verhaert, R.M.D.; Hilhorst, R.; Veeger, C.
    Enzyme kinetics in reversed micelles. 3. Behaviour of 20beta-hydroxysteroid dehydrogenase (1990), Eur. J. Biochem., 187, 81-88.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.53 0.0011 0.0025 progesterone in reversed micelles, value depends on type of detergent Streptomyces exfoliatus
1.1.1.53 0.0028 0.005 progesterone in aqueous solution, value increases with increasing ionic strength Streptomyces exfoliatus
1.1.1.53 0.0035 0.0057 NADH in aqueous solution, value increases with increasing ionic strength Streptomyces exfoliatus
1.1.1.53 0.004 0.016 NADH in reversed micelles, value depends on type of detergent Streptomyces exfoliatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.53 cytosol
-
 Streptomyces exfoliatus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.53 Streptomyces exfoliatus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.53 commercial preparation
-
 Streptomyces exfoliatus
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.53 -20°C, 5 mM phosphate buffer, pH 7.6 Streptomyces exfoliatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.53 progesterone + NAD(P)H
-
 Streptomyces exfoliatus 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.53 5.8
-
 reduction of progesterone in aqueous solution Streptomyces exfoliatus
1.1.1.53 6.4
-
 reduction of progesterone in reversed micelles Streptomyces exfoliatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.53 NADH
-
 Streptomyces exfoliatus