Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hayaishi, O.; Saito, Y.; Jakoby, W.B.; Stohlman, E.F.
    Reversible enzymatic oxidation of bile acids (1955), Arch. Biochem. Biophys., 56, 554-555.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.52 additional information SH-groups are required for activity Escherichia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.52 cytoplasm
-
 Escherichia sp. 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.52 3alpha-hydroxy-5beta-cholanate + NAD+ Escherichia sp.
-
 3-oxo-5beta-cholanate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.52 Escherichia sp.
-
 tentative identification: E. freundii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.52 partial Escherichia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.52 3alpha-hydroxy-5beta-cholanate + NAD+
-
 Escherichia sp. 3-oxo-5beta-cholanate + NADH
-
 r
1.1.1.52 3alpha-hydroxy-5beta-cholanate + NAD+ i.e. lithocholic acid Escherichia sp. 3-oxo-5beta-cholanate + NADH
-
 r
1.1.1.52 3alpha-hydroxy-bis-norcholanic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 3-oxo-bis-norcholanic acid + NADH
-
 r
1.1.1.52 3alpha-hydroxy-norcholanic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 3-oxo-norcholanic acid + NADH
-
 r
1.1.1.52 cholic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.52 dehydrocholic acid + NADH i.e. 3,7,12-trioxocholan-24-oic acid Escherichia sp. 3-hydroxy-7,12-dihydroxy-5beta-cholan-24-oic acid + NAD+
-
 r
1.1.1.52 deoxycholic acid + NAD+ oxidation at the same rate as hydroxycholanic acid Escherichia sp. 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 r
1.1.1.52 additional information no substrates: androsterone and tetrahydrocortisone, methylation of carboxyl group or reduction to alcohol inactivates Escherichia sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.52 26
-
 assay at Escherichia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.52 10.4
-
 oxidation, assay at Escherichia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.52 additional information
-
 oxidation proceeds at alkaline and reduction favorably at neutral pH-values Escherichia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.52 NAD+ NADP+ cannot replace NAD+ Escherichia sp.
1.1.1.52 NADH
-
 Escherichia sp.