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Literature summary extracted from

  • Heyns, W.; de Moor, P.
    A 3(17)beta-hydroxysteroid dehydrogenase in rat erythrocytes. Conversion of 5alpha-dihydrotestosterone into 5alpha-androstane-3beta,17beta-diol and purification of the enzyme by affinity chromatography (1974), Biochim. Biophys. Acta, 358, 1-13.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.51 Dimethylsulfoxide
-
 Rattus norvegicus
1.1.1.51 dioxane
-
 Rattus norvegicus
1.1.1.51 ethanol
-
 Rattus norvegicus
1.1.1.51 glycerol
-
 Rattus norvegicus
1.1.1.51 Propyleneglycol
-
 Rattus norvegicus
1.1.1.51 Triton X-100
-
 Rattus norvegicus
1.1.1.51 Tween-80
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.51 0.0018
-
 NADPH 5alpha-dihydrotestosterone as substrate, 3-beta-hydroxysteroid dehydrogenase activity Rattus norvegicus
1.1.1.51 0.0021
-
 NADPH estrone as substrate, 17beta-hydroxysteroid dehydrogenase activity Rattus norvegicus
1.1.1.51 0.0082
-
 NADP+ testosterone as substrate, 17beta-hydroxysteroid dehydrogenase activity Rattus norvegicus
1.1.1.51 0.009
-
 NADP+ 3beta-hydroxy-5alpha-androstan-17-one as substrate, 3beta-hydroxysteroid dehydrogenase activity Rattus norvegicus
1.1.1.51 0.14
-
 5alpha-dihydrotestosterone
-
 Rattus norvegicus
1.1.1.51 0.31
-
 testosterone
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.51 70000
-
 gel filtration Rattus norvegicus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.51 dimethylsulfoxide 3.3% v/v: inactivation 43% Rattus norvegicus
1.1.1.51 dioxane 3.3% v/v: inactivation 90% Rattus norvegicus
1.1.1.51 Ethanol 1.7% v/v: inactivation 22% Rattus norvegicus
1.1.1.51 Glycerol 5% v/v: inactivation 26% Rattus norvegicus
1.1.1.51 propyleneglycol 3.3% v/v: inactivation 50% Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 Rattus norvegicus
-
 Wistar
-
1.1.1.51 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.51
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.51 erythrocyte
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 3beta-hydroxy-5alpha-androstan-17-one + NADP+
-
 Rattus norvegicus 5alpha-androstane-3,17-dione + NADPH
-
 ?
1.1.1.51 5alpha-androstane-3beta,17beta-diol + NADP+
-
 Rattus norvegicus 3beta-hydroxy-5alpha-androstan-17-one + NADPH
-
 ?
1.1.1.51 5alpha-dihydrotestosterone + NAD(P)H
-
 Rattus norvegicus 5alpha-androstane-3beta,17beta-diol + NADP+
-
 ?
1.1.1.51 5alpha-dihydrotestosterone + NADP+
-
 Rattus norvegicus 5alpha-androstane-3,17-dione + NADPH + H+
-
 ?
1.1.1.51 5alpha-dihydrotestosterone + NADP+
-
 Rattus norvegicus Wistar 5alpha-androstane-3,17-dione + NADPH + H+
-
 ?
1.1.1.51 androst-5-ene-3beta,17beta-diol + NADP+
-
 Rattus norvegicus dehydroepiandrosterone + NADPH
-
 r
1.1.1.51 androst-5-ene-3beta,17beta-diol + NADP+
-
 Rattus norvegicus Wistar dehydroepiandrosterone + NADPH
-
 r
1.1.1.51 androsterone + NADPH + H+
-
 Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NADP+
-
 ?
1.1.1.51 androsterone + NADPH + H+
-
 Rattus norvegicus Wistar 5alpha-androstane-3alpha,17beta-diol + NADP+
-
 ?
1.1.1.51 estradiol + NADP+
-
 Rattus norvegicus estrone + NADPH
-
 r
1.1.1.51 estradiol + NADP+
-
 Rattus norvegicus Wistar estrone + NADPH
-
 r
1.1.1.51 testosterone + NAD(P)+
-
 Rattus norvegicus androst-4-en-3,17-dione + NAD(P)H + H+
-
 ?
1.1.1.51 testosterone + NAD(P)+
-
 Rattus norvegicus Wistar androst-4-en-3,17-dione + NAD(P)H + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.51 6 7 reductase activity Rattus norvegicus
1.1.1.51 8.2
-
 dehydrogenase activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.51 additional information
-
 unstable under slightly alkaline conditions Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.51 NADP+
-
 Rattus norvegicus
1.1.1.51 NADPH
-
 Rattus norvegicus