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Literature summary extracted from
- Purification and properties of glucose-6-phosphate dehydrogenase from Bacillus stearothermophilus (1985), J. Appl. Biochem., 7, 192-201.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.49 | additional information | - |
additional information | - |
Geobacillus stearothermophilus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | Mg2+ | activation | Geobacillus stearothermophilus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 52000 | - |
4 * 52000, SDS-PAGE | Geobacillus stearothermophilus |
| 1.1.1.49 | 200000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.49 | D-glucose 6-phosphate + H2O + NADP+ | Geobacillus stearothermophilus | - |
D-gluconate 6-phosphate + NADPH + H+ | 6-phosphoglucono-delta-lactone | r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.49 | Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.49 | - |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | additional information | - |
- |
Geobacillus stearothermophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.49 | 30°C, Bacillus stearothermophilus enzyme maintains more than 80% of activity after 1 month | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.49 | D-glucose 6-phosphate + H2O + NADP+ | - |
Geobacillus stearothermophilus | D-gluconate 6-phosphate + NADPH + H+ | 6-phosphoglucono-delta-lactone | r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.49 | tetramer | 4 * 52000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 30 | - |
1 month | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 8.5 | - |
- |
Geobacillus stearothermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | NAD+ | - |
Geobacillus stearothermophilus | |
| 1.1.1.49 | NADP+ | - |
Geobacillus stearothermophilus | |
| 1.1.1.49 | NADPH | - |
Geobacillus stearothermophilus | |
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Release 2023.1 (January 2023)
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