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Literature summary extracted from

  • Blachnitzky, E.O.; Wengenmayer, F.; Kurz, G.
    D-galactose dehydrogenase from Pseudomonas fluorescens. Purification, properties and structure (1974), Eur. J. Biochem., 47, 235-250.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.48 0.24
-
 NAD+
-
 Pseudomonas fluorescens
1.1.1.48 0.7
-
 D-galactose
-
 Pseudomonas fluorescens
1.1.1.48 2.3
-
 NADP+
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.48 32000
-
 alpha,alpha, 2 * 32000, SDS-PAGE Pseudomonas fluorescens
1.1.1.48 64000
-
 gel filtration Pseudomonas fluorescens
1.1.1.48 102000
-
 gel filtration Pelomonas saccharophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.48 D-galactose + NAD+ Pseudomonas fluorescens
-
 D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+ Pelomonas saccharophila
-
 D-galactono-1,5-lactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.48 Pelomonas saccharophila
-
-
-
1.1.1.48 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.48 ammonium sulfate, CM-Sephadex, DEAE-Sephadex, hydroxyapatite, disc electrophoresis Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.48 849
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.1.1.48 -20°C, 100 mM phosphate buffer, pH 6.5-7.6, 30-50% glycerol, 18 months, no loss of activity Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.48 2-deoxy-D-galactose + NAD+
-
 Pseudomonas fluorescens 2-deoxy-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 2-deoxy-D-galactose + NAD+
-
 Pelomonas saccharophila 2-deoxy-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 2-deoxy-D-galactose + NADP+
-
 Pseudomonas fluorescens 2-deoxy-galactono-1,5-lactone + NADPH
-
 ?
1.1.1.48 6-deoxy-alpha-D-galactose + NAD+
-
 Pseudomonas fluorescens 6-deoxy-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 6-deoxy-alpha-D-galactose + NAD+
-
 Pelomonas saccharophila 6-deoxy-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+
-
 Pseudomonas fluorescens D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 D-galactose + NAD+
-
 Pelomonas saccharophila D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 L-arabinose + NAD+
-
 Pseudomonas fluorescens L-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.48 L-arabinose + NAD+
-
 Pelomonas saccharophila L-arabinono-1,4-lactone + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.48 dimer alpha,alpha, 2 * 32000, SDS-PAGE Pseudomonas fluorescens
1.1.1.48 tetramer
-
 Pelomonas saccharophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.48 1180
-
 D-galactose
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.48 9.1 9.5
-
 Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.48 4.6 9.2
-
 Pseudomonas fluorescens