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Literature summary extracted from

  • Chooback, L.; Price, N.E.; Karsten, W.E.; Nelson, J.; Sundstrom, P.; Cook, P.F.
    Cloning, expression, purification, and characterization of the 6-phosphogluconate dehydrogenase from sheep liver (1998), Protein Expr. Purif., 13, 251-258.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.44 mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase from sheep liver reverse transcribed and amplified resulting cDNA subcloned into pQE-30 and overproduced in Escherichia coli M15 Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 51000
-
 2 * 51000, SDS-PAGE Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Escherichia coli
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Ovis aries
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Escherichia coli JM109 M15
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Cyberlindnera jadinii
-
-
-
1.1.1.44 Escherichia coli
-
 JM109, M15
-
1.1.1.44 Escherichia coli JM109 M15
-
 JM109, M15
-
1.1.1.44 Ovis aries
-
 sheep
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44 recombinant enzyme Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 4.7
-
 recombinant enzyme Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Escherichia coli D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Escherichia coli JM109 M15 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer
-
 Cyberlindnera jadinii
1.1.1.44 dimer
-
 Escherichia coli
1.1.1.44 dimer 2 * 51000, SDS-PAGE Ovis aries