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Literature summary extracted from

  • Sokolov, A.P.; Trotsenko, Y.A.
    Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from Arthrobacter globiformis (1990), Methods Enzymol., 188, 339-345.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 ATP
-
 Arthrobacter globiformis
1.1.1.44 Co2+
-
 Arthrobacter globiformis
1.1.1.44 Fe2+
-
 Arthrobacter globiformis
1.1.1.44 NADH
-
 Arthrobacter globiformis
1.1.1.44 NADPH
-
 Arthrobacter globiformis
1.1.1.44 Ni2+
-
 Arthrobacter globiformis
1.1.1.44 Zn2+
-
 Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.035
-
 NADP+
-
 Arthrobacter globiformis
1.1.1.44 0.11
-
 6-phosphogluconate
-
 Arthrobacter globiformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.44 Ca2+
-
 Arthrobacter globiformis
1.1.1.44 Mg2+
-
 Arthrobacter globiformis
1.1.1.44 Mn2+
-
 Arthrobacter globiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 57000
-
 4 * 57000, SDS-PAGE Arthrobacter globiformis
1.1.1.44 229000
-
 gel filtration Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Arthrobacter globiformis
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Arthrobacter globiformis VKM B-175
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Arthrobacter globiformis
-
 VKM B-175
-
1.1.1.44 Arthrobacter globiformis VKM B-175
-
 VKM B-175
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 Arthrobacter globiformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 25.7
-
-
 Arthrobacter globiformis

Storage Stability

EC Number Storage Stability Organism
1.1.1.44 4°C, purified enzyme in 50% glycerol buffer remains active for at least 3 months Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Arthrobacter globiformis D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Arthrobacter globiformis VKM B-175 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 tetramer 4 * 57000, SDS-PAGE Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+ absolutely specific with respect to NADP+, no reaction can be detected in the presence of NAD+ Arthrobacter globiformis