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Literature summary extracted from

  • Simcox, P.D.; Dennis, D.T.
    6-Phosphogluconate dehydrogenase isoenzymes from the developing endosperm of Ricinus communis L. (1978), Plant Physiol., 62, 287-290.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.44 both isoenzymes stabilized by the H2PO4- Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.0041
-
 NADP+ cytosolic isoenzyme Ricinus communis
1.1.1.44 0.0054
-
 NADP+ proplastid isoenzyme Ricinus communis
1.1.1.44 0.0096
-
 6-phosphogluconate proplastid isoenzyme Ricinus communis
1.1.1.44 0.012
-
 6-phosphogluconate cytosolic isoenzyme Ricinus communis
1.1.1.44 1.7
-
 MgCl2 cytosolic isoenzyme Ricinus communis
1.1.1.44 2.4
-
 MgCl2 proplastid isoenzyme Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.44 cytosol
-
 Spinacia oleracea 5829
-
1.1.1.44 cytosol
-
 Ricinus communis 5829
-
1.1.1.44 proplastid
-
 Ricinus communis 9537
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.44 Mg2+ MgCl2 stimulation for the oxidative decarboxylation of 6-phosphogluconate Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 54000
-
 2 * 54000, cytosolic isoenzyme, SDS-PAGE Ricinus communis
1.1.1.44 55000
-
 2 * 55000, SDS-PAGE Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Vigna radiata var. radiata
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Daucus carota
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Ricinus communis endosperm contains both cytosolic and plastic glycolytic and pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Spinacia oleracea enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Ricinus communis enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Daucus carota
-
 carrot
-
1.1.1.44 Ricinus communis
-
 castor bean, Baker 296 "Dwarf"
-
1.1.1.44 Spinacia oleracea
-
-
-
1.1.1.44 Vigna radiata var. radiata
-
 black gram, mung bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 endosperm
-
 Ricinus communis
-
1.1.1.44 leaf
-
 Spinacia oleracea
-
1.1.1.44 leaf
-
 Ricinus communis
-
1.1.1.44 root
-
 Daucus carota
-
1.1.1.44 seed
-
 Ricinus communis
-
1.1.1.44 seedling
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 13.6
-
-
 Ricinus communis
1.1.1.44 38
-
 cytoplasmic isoenzyme Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Vigna radiata var. radiata D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Spinacia oleracea D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ricinus communis D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Daucus carota D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ endosperm contains both cytosolic and plastic glycolytic and pentose phosphate pathway Ricinus communis D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Spinacia oleracea D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Ricinus communis D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer 2 * 55000, SDS-PAGE Ricinus communis
1.1.1.44 dimer 2 * 54000, cytosolic isoenzyme, SDS-PAGE Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 7.8 8 cytosolic and proplastid isoenzymes Ricinus communis