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Literature summary extracted from

  • Scott, W.A.; Abramsky, T.
    Neurospora 6-phosphogluconate dehydrogenase. I. Purification and characterization of the wild type enzyme (1973), J. Biol. Chem., 248, 3535-3544.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 D-fructose 1,6-diphosphate
-
 Neurospora crassa
1.1.1.44 D-fructose 1,6-diphosphate
-
 Ovis aries
1.1.1.44 iodoacetamide
-
 Neurospora crassa
1.1.1.44 NADPH
-
 Neurospora crassa
1.1.1.44 pyridoxal 5'-phosphate photooxidation Cyberlindnera jadinii
1.1.1.44 pyridoxal 5'-phosphate photooxidation Neurospora crassa
1.1.1.44 Rose bengal photooxidation Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.01
-
 6-phosphogluconate
-
 Neurospora crassa
1.1.1.44 0.025
-
 NADP+ source of enzyme: peak I Neurospora crassa
1.1.1.44 0.03
-
 NADP+ source of enzyme: whole cells Neurospora crassa
1.1.1.44 0.033
-
 NADP+ source of enzyme: peak II Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 57000
-
 2 * 57000, SDS-PAGE Neurospora crassa
1.1.1.44 110000 120000 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Neurospora crassa enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Ovis aries enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Cyberlindnera jadinii
-
-
-
1.1.1.44 Neurospora crassa
-
-
-
1.1.1.44 Ovis aries
-
 sheep
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 30.8
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer
-
 Cyberlindnera jadinii
1.1.1.44 dimer
-
 Ovis aries
1.1.1.44 dimer 2 * 57000, SDS-PAGE Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.44 50
-
 stable for at least 120 min Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 7.8 9
-
 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 3-acetylpyridine-NADP+
-
 Neurospora crassa
1.1.1.44 deamino-NADP+
-
 Neurospora crassa