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Literature summary extracted from

  • Frampton, E.W.; Wood, W.A.
    Carbohydrate oxidation by Pseudomonas fluorescens VI. Conversion of 2-keto-6-phosphogluconate to pyruvate (1961), J. Biol. Chem., 236, 2571-2577.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.43 NaF
-
 Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.43 0.38
-
 2-keto-6-phospho-D-gluconate with NADPH as coenzyme Pseudomonas fluorescens
1.1.1.43 0.66
-
 2-keto-6-phospho-D-gluconate with NADH as coenzyme Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Saccharomyces cerevisiae
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Enterobacter cloacae
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Pseudomonas fluorescens
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Leuconostoc mesenteroides
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Pseudomonas fluorescens A 3.12
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.43 Enterobacter cloacae
-
-
-
1.1.1.43 Leuconostoc mesenteroides
-
-
-
1.1.1.43 Pseudomonas fluorescens
-
-
-
1.1.1.43 Pseudomonas fluorescens A 3.12
-
-
-
1.1.1.43 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.43
-
 Saccharomyces cerevisiae
1.1.1.43
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.43 98.6
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.1.1.43 0°C freezing preserves activity for several days Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.43 2-ketogluconate + NAD(P)H
-
 Pseudomonas fluorescens D-gluconate + NAD(P)+
-
 r
1.1.1.43 2-ketogluconate + NAD(P)H
-
 Pseudomonas fluorescens A 3.12 D-gluconate + NAD(P)+
-
 r
1.1.1.43 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 Enterobacter cloacae 6-phospho-D-gluconate + NAD(P)+
-
 r
1.1.1.43 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 Pseudomonas fluorescens 6-phospho-D-gluconate + NAD(P)+
-
 r
1.1.1.43 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 Leuconostoc mesenteroides 6-phospho-D-gluconate + NAD(P)+
-
 r
1.1.1.43 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 Pseudomonas fluorescens A 3.12 6-phospho-D-gluconate + NAD(P)+
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Saccharomyces cerevisiae 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Enterobacter cloacae 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas fluorescens 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Leuconostoc mesenteroides 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas fluorescens A 3.12 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 additional information fructose-6-phosphate and 2-keto-3-deoxy-6-phosphogluconate are not reduced Pseudomonas fluorescens ?
-
 ?
1.1.1.43 additional information fructose-6-phosphate and 2-keto-3-deoxy-6-phosphogluconate are not reduced Pseudomonas fluorescens A 3.12 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.43 7.2 8 coenzyme NADH Pseudomonas fluorescens
1.1.1.43 7.4
-
 coenzyme NADPH Pseudomonas fluorescens