BRENDA - Enzyme Database

Purification and characterization of chloroplastic NADP-isocitrate dehydrogenase from mixotrophic tobacco cells

Galvez, S.; Bismuth, E.; Sarda, C.; Gadal, P.; Plant Physiol. 105, 593-600 (1994)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.007
-
NADP+
Mg2+-dependent reaction, ICDH2
Nicotiana tabacum
1.1.1.42
0.0075
-
NADP+
Mg2+-dependent reaction, ICDH1
Nicotiana tabacum
1.1.1.42
0.012
-
Mg2+
Mg2+-dependent reaction, ICDH2
Nicotiana tabacum
1.1.1.42
0.013
-
Mg2+
Mg2+-dependent reaction, ICDH1
Nicotiana tabacum
1.1.1.42
0.028
-
D-isocitrate
Mg2+-dependent reaction, ICDH2
Nicotiana tabacum
1.1.1.42
0.041
-
D-isocitrate
Mg2+-dependent reaction, ICDH1
Nicotiana tabacum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
chloroplast
ICDH2
Nicotiana tabacum
9507
-
1.1.1.42
cytosol
ICDH1
Nicotiana tabacum
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
117000
-
gel filtration, ICDH1
Nicotiana tabacum
1.1.1.42
136000
-
gel filtration, ICDH2
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Nicotiana tabacum
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Nicotiana tabacum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Nicotiana tabacum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
leaf
-
Nicotiana tabacum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
18.6
-
ICDH2
Nicotiana tabacum
1.1.1.42
50
-
ICDH1
Nicotiana tabacum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286810
Nicotiana tabacum
2-oxoglutarate + NADPH + CO2
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Nicotiana tabacum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Nicotiana tabacum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.007
-
NADP+
Mg2+-dependent reaction, ICDH2
Nicotiana tabacum
1.1.1.42
0.0075
-
NADP+
Mg2+-dependent reaction, ICDH1
Nicotiana tabacum
1.1.1.42
0.012
-
Mg2+
Mg2+-dependent reaction, ICDH2
Nicotiana tabacum
1.1.1.42
0.013
-
Mg2+
Mg2+-dependent reaction, ICDH1
Nicotiana tabacum
1.1.1.42
0.028
-
D-isocitrate
Mg2+-dependent reaction, ICDH2
Nicotiana tabacum
1.1.1.42
0.041
-
D-isocitrate
Mg2+-dependent reaction, ICDH1
Nicotiana tabacum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
chloroplast
ICDH2
Nicotiana tabacum
9507
-
1.1.1.42
cytosol
ICDH1
Nicotiana tabacum
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
117000
-
gel filtration, ICDH1
Nicotiana tabacum
1.1.1.42
136000
-
gel filtration, ICDH2
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Nicotiana tabacum
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Nicotiana tabacum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
leaf
-
Nicotiana tabacum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
18.6
-
ICDH2
Nicotiana tabacum
1.1.1.42
50
-
ICDH1
Nicotiana tabacum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286810
Nicotiana tabacum
2-oxoglutarate + NADPH + CO2
-
-
-
?