BRENDA - Enzyme Database

Characterization of a heat-stable NADP-dependent isocitrate dehydrogenase from the obligate thermophile Thermoleophilum minutum YS-4

Novotny, J.F.; Perry, J.J.; Appl. Microbiol. Biotechnol. 35, 461-465 (1991)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
p-chloromercuribenzoate
-
Thermoleophilum minutum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.033
-
D,L-isocitrate
-
Thermoleophilum minutum
1.1.1.42
0.048
-
NADP+
-
Thermoleophilum minutum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Thermoleophilum minutum
1.1.1.42
Mn2+
absolute requirement for divalent cations
Thermoleophilum minutum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
30500
-
alpha2, 2 * 30500, SDS-PAGE
Thermoleophilum minutum
1.1.1.42
60000
-
PAGE and gel filtration
Thermoleophilum minutum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermoleophilum minutum
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Thermoleophilum minutum YS-4
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Thermoleophilum minutum
-
YS-4
-
1.1.1.42
Thermoleophilum minutum YS-4
-
YS-4
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Thermoleophilum minutum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
3884
-
-
Thermoleophilum minutum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286809
Thermoleophilum minutum
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286809
Thermoleophilum minutum YS-4
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 30500, SDS-PAGE
Thermoleophilum minutum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
75
-
-
Thermoleophilum minutum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
70
-
after 1 h incubation retains 57% of initial activity
Thermoleophilum minutum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.2
-
-
Thermoleophilum minutum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Thermoleophilum minutum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Thermoleophilum minutum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
p-chloromercuribenzoate
-
Thermoleophilum minutum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.033
-
D,L-isocitrate
-
Thermoleophilum minutum
1.1.1.42
0.048
-
NADP+
-
Thermoleophilum minutum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Thermoleophilum minutum
1.1.1.42
Mn2+
absolute requirement for divalent cations
Thermoleophilum minutum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
30500
-
alpha2, 2 * 30500, SDS-PAGE
Thermoleophilum minutum
1.1.1.42
60000
-
PAGE and gel filtration
Thermoleophilum minutum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermoleophilum minutum
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Thermoleophilum minutum YS-4
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Thermoleophilum minutum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
3884
-
-
Thermoleophilum minutum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286809
Thermoleophilum minutum
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286809
Thermoleophilum minutum YS-4
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 30500, SDS-PAGE
Thermoleophilum minutum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
75
-
-
Thermoleophilum minutum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
70
-
after 1 h incubation retains 57% of initial activity
Thermoleophilum minutum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.2
-
-
Thermoleophilum minutum