BRENDA - Enzyme Database

Purification and properties of cytosolic NADP-isocitrate dehydrogenase from Chlamydomonas reinhardtii

Martinez-Rivas, J.M.; Garcia-Diaz, M.T.; Vega, J.M.; Plant Physiol. Biochem. 34, 673-676 (1996)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2,3-Butanedione
-
Chlamydomonas reinhardtii
1.1.1.42
Phenylglyoxal
-
Chlamydomonas reinhardtii
1.1.1.42
pyridoxal 5'-phosphate
-
Chlamydomonas reinhardtii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Chlamydomonas reinhardtii
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mn2+
-
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
40000
-
alpha2, 2 * 40000, SDS-PAGE
Chlamydomonas reinhardtii
1.1.1.42
70000
-
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Chlamydomonas reinhardtii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286806
Chlamydomonas reinhardtii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 40000, SDS-PAGE
Chlamydomonas reinhardtii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
45
-
after 10 min completely inactivated in absence of substrate
Chlamydomonas reinhardtii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Chlamydomonas reinhardtii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2,3-Butanedione
-
Chlamydomonas reinhardtii
1.1.1.42
Phenylglyoxal
-
Chlamydomonas reinhardtii
1.1.1.42
pyridoxal 5'-phosphate
-
Chlamydomonas reinhardtii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Chlamydomonas reinhardtii
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mn2+
-
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
40000
-
alpha2, 2 * 40000, SDS-PAGE
Chlamydomonas reinhardtii
1.1.1.42
70000
-
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Chlamydomonas reinhardtii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286806
Chlamydomonas reinhardtii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 40000, SDS-PAGE
Chlamydomonas reinhardtii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
45
-
after 10 min completely inactivated in absence of substrate
Chlamydomonas reinhardtii