BRENDA - Enzyme Database

Structural constrains in protein engineering. The coenzyme specificity of Escherichia coli isocitrate dehydrogenase

Chen, R.; Greer, A.F.; Dean, A.M.; Eur. J. Biochem. 250, 578-582 (1997)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
C201M
higher affinity for NAD+ than for NADP+
Escherichia coli
1.1.1.42
C201N
higher affinity for NAD+ than for NADP+
Escherichia coli
1.1.1.42
C201V
higher affinity for NAD+ than for NADP+
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Escherichia coli
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286804
Escherichia coli
2-oxoglutarate + NADPH + CO2
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
C201M
higher affinity for NAD+ than for NADP+
Escherichia coli
1.1.1.42
C201N
higher affinity for NAD+ than for NADP+
Escherichia coli
1.1.1.42
C201V
higher affinity for NAD+ than for NADP+
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Escherichia coli
-
2-oxoglutarate + NADPH + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286804
Escherichia coli
2-oxoglutarate + NADPH + CO2
-
-
-
?