BRENDA - Enzyme Database

Purification and properties of NADP-isocitrate dehydrogenase from the unicellular cyanobacterium Synechocystis sp. PCC 6803

Muro-Pastor, M.I.; Florencio, F.J.; Eur. J. Biochem. 203, 99-105 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
non-competitive inhibitor
Synechocystis sp.
1.1.1.42
5'-ADP
non-competitive inhibitor
Synechocystis sp.
1.1.1.42
NADPH
competitive inhibitor
Synechocystis sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.004
-
Mn2+
Mn2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.0057
-
D,L-isocitrate
Mn2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.006
-
NADP+
Mn2+-dependent raction
Synechocystis sp.
1.1.1.42
0.012
-
NADP+
Mg2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.059
-
D,L-isocitrate
Mg2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.125
-
Mg2+
Mg2+-dependent reaction
Synechocystis sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Synechocystis sp.
1.1.1.42
Mn2+
absolute requirement for divalent cations
Synechocystis sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
57000
-
alpha2, 2 * 57000, SDS-PAGE
Synechocystis sp.
1.1.1.42
108000
-
gel filtration
Synechocystis sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Synechocystis sp.
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Synechocystis sp.
-
PCC 6803
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Synechocystis sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
15.7
-
-
Synechocystis sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286803
Synechocystis sp.
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 57000, SDS-PAGE
Synechocystis sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
9
-
-
Synechocystis sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
7.5
10
-
Synechocystis sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Synechocystis sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Synechocystis sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
non-competitive inhibitor
Synechocystis sp.
1.1.1.42
5'-ADP
non-competitive inhibitor
Synechocystis sp.
1.1.1.42
NADPH
competitive inhibitor
Synechocystis sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.004
-
Mn2+
Mn2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.0057
-
D,L-isocitrate
Mn2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.006
-
NADP+
Mn2+-dependent raction
Synechocystis sp.
1.1.1.42
0.012
-
NADP+
Mg2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.059
-
D,L-isocitrate
Mg2+-dependent reaction
Synechocystis sp.
1.1.1.42
0.125
-
Mg2+
Mg2+-dependent reaction
Synechocystis sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Synechocystis sp.
1.1.1.42
Mn2+
absolute requirement for divalent cations
Synechocystis sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
57000
-
alpha2, 2 * 57000, SDS-PAGE
Synechocystis sp.
1.1.1.42
108000
-
gel filtration
Synechocystis sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Synechocystis sp.
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Synechocystis sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
15.7
-
-
Synechocystis sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286803
Synechocystis sp.
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 57000, SDS-PAGE
Synechocystis sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
9
-
-
Synechocystis sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
7.5
10
-
Synechocystis sp.