BRENDA - Enzyme Database

Purification and characterization of a monomeric isocitrate dehydrogenase with dual coenzyme specificity from the photosynthetic bacterium Rhodomicrobium vannielii

Leyland, M.L.; Kelly, D.J.; Eur. J. Biochem. 202, 85-93 (1991)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0025
-
NADP+
-
Rhodomicrobium vannielii
1.1.1.42
0.77
-
NAD+
-
Rhodomicrobium vannielii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
75000
80000
PAGE
Rhodomicrobium vannielii
1.1.1.42
80000
-
1 * 80000, SDS-PAGE
Rhodomicrobium vannielii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Rhodomicrobium vannielii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Rhodomicrobium vannielii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Rhodomicrobium vannielii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
18.5
-
NAD+-linked reaction
Rhodomicrobium vannielii
1.1.1.42
23
-
NADP+-linked reaction
Rhodomicrobium vannielii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286802
Rhodomicrobium vannielii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
monomer
1 * 80000, SDS-PAGE
Rhodomicrobium vannielii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NAD+
-
Rhodomicrobium vannielii
1.1.1.42
NADP+
-
Rhodomicrobium vannielii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NAD+
-
Rhodomicrobium vannielii
1.1.1.42
NADP+
-
Rhodomicrobium vannielii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0025
-
NADP+
-
Rhodomicrobium vannielii
1.1.1.42
0.77
-
NAD+
-
Rhodomicrobium vannielii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
75000
80000
PAGE
Rhodomicrobium vannielii
1.1.1.42
80000
-
1 * 80000, SDS-PAGE
Rhodomicrobium vannielii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Rhodomicrobium vannielii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Rhodomicrobium vannielii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
18.5
-
NAD+-linked reaction
Rhodomicrobium vannielii
1.1.1.42
23
-
NADP+-linked reaction
Rhodomicrobium vannielii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286802
Rhodomicrobium vannielii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
monomer
1 * 80000, SDS-PAGE
Rhodomicrobium vannielii