BRENDA - Enzyme Database

Structural basis for a change in substrate specificity: crystal structur of S113E isocitrate dehydrogenase in a complex with isopropylmalate, Mg2+, and NAPD

Doyle, S.A.; Beernink, B.T.; Koshland, Jr., D.E.; Biochemistry 40, 4234-4241 (2001)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.42
S113E
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
S113E
affinity for isopropylmalate is 37-fold compared to wild-type
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Escherichia coli
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286801
Escherichia coli
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isopropylmalate + NADP+
-
286801
Escherichia coli
?
-
-
-
?
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.42
S113E
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
S113E
affinity for isopropylmalate is 37-fold compared to wild-type
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Escherichia coli
-
2-oxoglutarate + NADPH + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286801
Escherichia coli
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isopropylmalate + NADP+
-
286801
Escherichia coli
?
-
-
-
?