BRENDA - Enzyme Database

Purification and characterization of isocitrate dehydrogenase from a hyperthermophilic archaebacterium, Caldococcus noboribetus

Aoshima, M.; Oshima, T.; Biochim. Biophys. Acta 1340, 227-234 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
expression in Escherichia coli
Caldococcus noboribetus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.043
-
NADP+
-
Caldococcus noboribetus
1.1.1.42
0.072
-
D,L-isocitrate
-
Caldococcus noboribetus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Caldococcus noboribetus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Caldococcus noboribetus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
48000
-
alpha2, 2 * 48000, SDS-PAGE
Caldococcus noboribetus
1.1.1.42
90000
-
gel filtration
Caldococcus noboribetus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Caldococcus noboribetus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Caldococcus noboribetus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Caldococcus noboribetus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
144
-
-
Caldococcus noboribetus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286798
Caldococcus noboribetus
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 48000, SDS-PAGE
Caldococcus noboribetus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
40
-
nearly no activity at 40°C or lower
Caldococcus noboribetus
1.1.1.42
80
-
no loss in activity
Caldococcus noboribetus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7
-
-
Caldococcus noboribetus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
expression in Escherichia coli
Caldococcus noboribetus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.043
-
NADP+
-
Caldococcus noboribetus
1.1.1.42
0.072
-
D,L-isocitrate
-
Caldococcus noboribetus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Caldococcus noboribetus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Caldococcus noboribetus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
48000
-
alpha2, 2 * 48000, SDS-PAGE
Caldococcus noboribetus
1.1.1.42
90000
-
gel filtration
Caldococcus noboribetus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Caldococcus noboribetus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Caldococcus noboribetus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
144
-
-
Caldococcus noboribetus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286798
Caldococcus noboribetus
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 48000, SDS-PAGE
Caldococcus noboribetus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
40
-
nearly no activity at 40°C or lower
Caldococcus noboribetus
1.1.1.42
80
-
no loss in activity
Caldococcus noboribetus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7
-
-
Caldococcus noboribetus