BRENDA - Enzyme Database

Purification and characterization of NADP+-linked isocitrate dehydrogenase from an alkalophilic Bacillus

Shikata, S.; Ozaki, K.; Kawai, S.; Ito, S.; Okamoto, K.; Biochim. Biophys. Acta 952, 282-289 (1988)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
3-phosphoglycerate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
ATP
-
Bacillus sp. (in: Bacteria)
1.1.1.42
cis-aconitate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
Cu2+
-
Bacillus sp. (in: Bacteria)
1.1.1.42
glyceraldehyde-3-phosphate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
Hg2+
-
Bacillus sp. (in: Bacteria)
1.1.1.42
N-ethylmaleimide
-
Bacillus sp. (in: Bacteria)
1.1.1.42
NADPH
-
Bacillus sp. (in: Bacteria)
1.1.1.42
oxaloacetate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
p-chloromercuribenzoate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
phosphoenolpyruvate
-
Bacillus sp. (in: Bacteria)
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0073
-
NADP+
-
Bacillus sp. (in: Bacteria)
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Cd2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Co2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Mg2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Mn2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Zn2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
90000
-
gel filtration
Bacillus sp. (in: Bacteria)
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Bacillus sp. (in: Bacteria)
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Bacillus sp. (in: Bacteria)
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286796
Bacillus sp. (in: Bacteria)
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
75
-
-
Bacillus sp. (in: Bacteria)
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
45
-
-
Bacillus sp. (in: Bacteria)
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.9
8.4
-
Bacillus sp. (in: Bacteria)
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
8
10
-
Bacillus sp. (in: Bacteria)
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Bacillus sp. (in: Bacteria)
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Bacillus sp. (in: Bacteria)
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
3-phosphoglycerate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
ATP
-
Bacillus sp. (in: Bacteria)
1.1.1.42
cis-aconitate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
Cu2+
-
Bacillus sp. (in: Bacteria)
1.1.1.42
glyceraldehyde-3-phosphate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
Hg2+
-
Bacillus sp. (in: Bacteria)
1.1.1.42
N-ethylmaleimide
-
Bacillus sp. (in: Bacteria)
1.1.1.42
NADPH
-
Bacillus sp. (in: Bacteria)
1.1.1.42
oxaloacetate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
p-chloromercuribenzoate
-
Bacillus sp. (in: Bacteria)
1.1.1.42
phosphoenolpyruvate
-
Bacillus sp. (in: Bacteria)
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0073
-
NADP+
-
Bacillus sp. (in: Bacteria)
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Cd2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Co2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Mg2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Mn2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
1.1.1.42
Zn2+
absolute requirement for divalent cations
Bacillus sp. (in: Bacteria)
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
90000
-
gel filtration
Bacillus sp. (in: Bacteria)
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Bacillus sp. (in: Bacteria)
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286796
Bacillus sp. (in: Bacteria)
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
75
-
-
Bacillus sp. (in: Bacteria)
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
45
-
-
Bacillus sp. (in: Bacteria)
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.9
8.4
-
Bacillus sp. (in: Bacteria)
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
8
10
-
Bacillus sp. (in: Bacteria)