Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dhariwal, K.R.; Venkitasubramanian, T.A.
    NADP-specific isocitrate dehydrogenase of Mycobacterium phlei ATCC 354: purification and characterization (1987), J. Gen. Microbiol., 133, 2457-2460.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 2-mercaptoethanol
-
 Mycolicibacterium phlei
1.1.1.42 dithiothreitol
-
 Mycolicibacterium phlei
1.1.1.42 glutathione
-
 Mycolicibacterium phlei
1.1.1.42 glyoxylate plus oxaloacetate Mycolicibacterium phlei
1.1.1.42 Hg2+
-
 Mycolicibacterium phlei
1.1.1.42 monoiodoacetate
-
 Mycolicibacterium phlei
1.1.1.42 Oxalomalate
-
 Mycolicibacterium phlei
1.1.1.42 p-chloromercuribenzoate
-
 Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.053
-
 NADP+
-
 Mycolicibacterium phlei
1.1.1.42 0.074
-
 D,L-isocitrate
-
 Mycolicibacterium phlei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ absolute requirement for divalent cations Crithidia fasciculata
1.1.1.42 Mg2+ absolute requirement for divalent cations Mycolicibacterium phlei
1.1.1.42 Mg2+ absolute requirement for divalent cations [Brevibacterium] flavum
1.1.1.42 Mg2+ absolute requirement for divalent cations Starkeya novella
1.1.1.42 Mn2+ absolute requirement for divalent cations Crithidia fasciculata
1.1.1.42 Mn2+ absolute requirement for divalent cations Mycolicibacterium phlei
1.1.1.42 Mn2+ absolute requirement for divalent cations [Brevibacterium] flavum
1.1.1.42 Mn2+ absolute requirement for divalent cations Starkeya novella
1.1.1.42 Zn2+ absolute requirement for divalent cations Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Crithidia fasciculata
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Mycolicibacterium phlei
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ [Brevibacterium] flavum
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Starkeya novella
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Crithidia fasciculata
-
-
-
1.1.1.42 Mycolicibacterium phlei
-
-
-
1.1.1.42 Starkeya novella
-
-
-
1.1.1.42 [Brevibacterium] flavum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
 Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 109
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Crithidia fasciculata 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Mycolicibacterium phlei 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 [Brevibacterium] flavum 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Starkeya novella 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 25 30
-
 Mycolicibacterium phlei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 8.5
-
-
 Mycolicibacterium phlei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.42 6.5 10
-
 Mycolicibacterium phlei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Crithidia fasciculata
1.1.1.42 NADP+
-
 Mycolicibacterium phlei
1.1.1.42 NADP+
-
 [Brevibacterium] flavum
1.1.1.42 NADP+
-
 Starkeya novella