BRENDA - Enzyme Database

NADP-specific isocitrate dehydrogenase of Mycobacterium phlei ATCC 354: purification and characterization

Dhariwal, K.R.; Venkitasubramanian, T.A.; J. Gen. Microbiol. 133, 2457-2460 (1987)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-mercaptoethanol
-
Mycolicibacterium phlei
1.1.1.42
dithiothreitol
-
Mycolicibacterium phlei
1.1.1.42
glutathione
-
Mycolicibacterium phlei
1.1.1.42
glyoxylate
plus oxaloacetate
Mycolicibacterium phlei
1.1.1.42
Hg2+
-
Mycolicibacterium phlei
1.1.1.42
monoiodoacetate
-
Mycolicibacterium phlei
1.1.1.42
Oxalomalate
-
Mycolicibacterium phlei
1.1.1.42
p-chloromercuribenzoate
-
Mycolicibacterium phlei
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.053
-
NADP+
-
Mycolicibacterium phlei
1.1.1.42
0.074
-
D,L-isocitrate
-
Mycolicibacterium phlei
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Brevibacterium flavum
1.1.1.42
Mg2+
absolute requirement for divalent cations
Crithidia fasciculata
1.1.1.42
Mg2+
absolute requirement for divalent cations
Mycolicibacterium phlei
1.1.1.42
Mg2+
absolute requirement for divalent cations
Starkeya novella
1.1.1.42
Mn2+
absolute requirement for divalent cations
Brevibacterium flavum
1.1.1.42
Mn2+
absolute requirement for divalent cations
Crithidia fasciculata
1.1.1.42
Mn2+
absolute requirement for divalent cations
Mycolicibacterium phlei
1.1.1.42
Mn2+
absolute requirement for divalent cations
Starkeya novella
1.1.1.42
Zn2+
absolute requirement for divalent cations
Mycolicibacterium phlei
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Crithidia fasciculata
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Mycolicibacterium phlei
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Brevibacterium flavum
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Starkeya novella
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Brevibacterium flavum
-
-
-
1.1.1.42
Crithidia fasciculata
-
-
-
1.1.1.42
Mycolicibacterium phlei
-
-
-
1.1.1.42
Starkeya novella
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Mycolicibacterium phlei
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
109
-
-
Mycolicibacterium phlei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286795
Crithidia fasciculata
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286795
Mycolicibacterium phlei
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286795
Brevibacterium flavum
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286795
Starkeya novella
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
25
30
-
Mycolicibacterium phlei
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8.5
-
-
Mycolicibacterium phlei
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6.5
10
-
Mycolicibacterium phlei
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Brevibacterium flavum
1.1.1.42
NADP+
-
Crithidia fasciculata
1.1.1.42
NADP+
-
Mycolicibacterium phlei
1.1.1.42
NADP+
-
Starkeya novella
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Brevibacterium flavum
1.1.1.42
NADP+
-
Crithidia fasciculata
1.1.1.42
NADP+
-
Mycolicibacterium phlei
1.1.1.42
NADP+
-
Starkeya novella
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-mercaptoethanol
-
Mycolicibacterium phlei
1.1.1.42
dithiothreitol
-
Mycolicibacterium phlei
1.1.1.42
glutathione
-
Mycolicibacterium phlei
1.1.1.42
glyoxylate
plus oxaloacetate
Mycolicibacterium phlei
1.1.1.42
Hg2+
-
Mycolicibacterium phlei
1.1.1.42
monoiodoacetate
-
Mycolicibacterium phlei
1.1.1.42
Oxalomalate
-
Mycolicibacterium phlei
1.1.1.42
p-chloromercuribenzoate
-
Mycolicibacterium phlei
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.053
-
NADP+
-
Mycolicibacterium phlei
1.1.1.42
0.074
-
D,L-isocitrate
-
Mycolicibacterium phlei
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Brevibacterium flavum
1.1.1.42
Mg2+
absolute requirement for divalent cations
Crithidia fasciculata
1.1.1.42
Mg2+
absolute requirement for divalent cations
Mycolicibacterium phlei
1.1.1.42
Mg2+
absolute requirement for divalent cations
Starkeya novella
1.1.1.42
Mn2+
absolute requirement for divalent cations
Brevibacterium flavum
1.1.1.42
Mn2+
absolute requirement for divalent cations
Crithidia fasciculata
1.1.1.42
Mn2+
absolute requirement for divalent cations
Mycolicibacterium phlei
1.1.1.42
Mn2+
absolute requirement for divalent cations
Starkeya novella
1.1.1.42
Zn2+
absolute requirement for divalent cations
Mycolicibacterium phlei
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Crithidia fasciculata
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Mycolicibacterium phlei
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Brevibacterium flavum
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Starkeya novella
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Mycolicibacterium phlei
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
109
-
-
Mycolicibacterium phlei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286795
Crithidia fasciculata
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286795
Mycolicibacterium phlei
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286795
Brevibacterium flavum
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286795
Starkeya novella
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
25
30
-
Mycolicibacterium phlei
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8.5
-
-
Mycolicibacterium phlei
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6.5
10
-
Mycolicibacterium phlei