BRENDA - Enzyme Database

NADP-specific isocitrate dehydrogenase from the citric acid-accumulating fungus Aspergillus niger

Meixner-Monori, B.; Kubicek, C.P.; Harrer, W.; Schreferl, G.; Rohe, M.; Biochem. J. 236, 549-557 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
substrate inhibition
Aspergillus niger
1.1.1.42
ATP
-
Aspergillus niger
1.1.1.42
citrate
-
Aspergillus niger
1.1.1.42
glyoxylate
plus oxaloacetate
Aspergillus niger
1.1.1.42
NADPH
-
Aspergillus niger
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytoplasm
-
Aspergillus niger
5737
-
1.1.1.42
cytoplasm
-
Blastocladiella emersonii
5737
-
1.1.1.42
cytoplasm
-
Phycomyces blakesleeanus
5737
-
1.1.1.42
mitochondrion
-
Aspergillus niger
5739
-
1.1.1.42
mitochondrion
-
Blastocladiella emersonii
5739
-
1.1.1.42
mitochondrion
-
Phycomyces blakesleeanus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Aspergillus niger
1.1.1.42
Mn2+
absolute requirement for divalent cations
Aspergillus niger
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
60000
-
PAGE
Aspergillus niger
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Aspergillus niger
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Phycomyces blakesleeanus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Blastocladiella emersonii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Aspergillus niger
-
-
-
1.1.1.42
Blastocladiella emersonii
-
-
-
1.1.1.42
Phycomyces blakesleeanus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Aspergillus niger
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
14.4
-
-
Aspergillus niger
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286793
Aspergillus niger
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286793
Phycomyces blakesleeanus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286793
Blastocladiella emersonii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.1.1.42
28
-
-
Aspergillus niger
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6
8
-
Aspergillus niger
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6.3
7.8
-
Aspergillus niger
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Blastocladiella emersonii
1.1.1.42
NADP+
-
Phycomyces blakesleeanus
1.1.1.42
NADP+
-
Aspergillus niger
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Blastocladiella emersonii
1.1.1.42
NADP+
-
Phycomyces blakesleeanus
1.1.1.42
NADP+
-
Aspergillus niger
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
substrate inhibition
Aspergillus niger
1.1.1.42
ATP
-
Aspergillus niger
1.1.1.42
citrate
-
Aspergillus niger
1.1.1.42
glyoxylate
plus oxaloacetate
Aspergillus niger
1.1.1.42
NADPH
-
Aspergillus niger
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytoplasm
-
Aspergillus niger
5737
-
1.1.1.42
cytoplasm
-
Blastocladiella emersonii
5737
-
1.1.1.42
cytoplasm
-
Phycomyces blakesleeanus
5737
-
1.1.1.42
mitochondrion
-
Aspergillus niger
5739
-
1.1.1.42
mitochondrion
-
Blastocladiella emersonii
5739
-
1.1.1.42
mitochondrion
-
Phycomyces blakesleeanus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Aspergillus niger
1.1.1.42
Mn2+
absolute requirement for divalent cations
Aspergillus niger
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
60000
-
PAGE
Aspergillus niger
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Aspergillus niger
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Phycomyces blakesleeanus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Blastocladiella emersonii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Aspergillus niger
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
14.4
-
-
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286793
Aspergillus niger
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286793
Phycomyces blakesleeanus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286793
Blastocladiella emersonii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.1.1.42
28
-
-
Aspergillus niger
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6
8
-
Aspergillus niger
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6.3
7.8
-
Aspergillus niger